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Titolo:
Adequacy of in situ glass slides and direct sand extractions to assess themicrobiota within sand columns used for drinking water treatment
Autore:
Langmark, J; Ashbolt, NJ; Szewzyk, U; Stenstrom, TA;
Indirizzi:
Univ New S Wales, Sch Civil & Environm Engn, Sydney, NSW 2052, Australia Univ New S Wales Sydney NSW Australia 2052 n, Sydney, NSW 2052, Australia Swedish Inst Infect Dis Control, S-17182 Solna, Sweden Swedish Inst InfectDis Control Solna Sweden S-17182 17182 Solna, Sweden Tech Univ Berlin, Dept Microbiol, D-10587 Berlin, Germany Tech Univ Berlin Berlin Germany D-10587 crobiol, D-10587 Berlin, Germany
Titolo Testata:
CANADIAN JOURNAL OF MICROBIOLOGY
fascicolo: 7, volume: 47, anno: 2001,
pagine: 601 - 607
SICI:
0008-4166(200107)47:7<601:AOISGS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TARGETED OLIGONUCLEOTIDE PROBES; BACTERIAL COMMUNITY STRUCTURE; RIBOSOMAL-RNA GENES; LABELED OLIGONUCLEOTIDES; INSITU HYBRIDIZATION; DIRECT ENUMERATION; MICROORGANISMS; SOIL; IDENTIFICATION; POPULATIONS;
Keywords:
sand filter; biofilms; in situ hybridization; groundwater recharge;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Ashbolt, NJ Univ New S Wales, Sch Civil & Environm Engn, Sydney, NSW 2052,Australia Univ New S Wales Sydney NSW Australia 2052 SW 2052, Australia
Citazione:
J. Langmark et al., "Adequacy of in situ glass slides and direct sand extractions to assess themicrobiota within sand columns used for drinking water treatment", CAN J MICRO, 47(7), 2001, pp. 601-607

Abstract

Historically, Cholodny-Rossi buried glass slide techniques have been used to study the microbiota of subsurface environments, yet the bias of such a technique has not been compared against direct sand extraction using modernin situ probing. Over a period of 34 wk, four separate 4-m-deep sand columns receiving raw lake water were examined to compare direct extraction of sand filter biofilm material against in situ glass slide biofilms. Significantly different DAPI direct counts and fluorescent in situ hybridization signals for major phylogenetic groups were observed. Not only were lower proportions (P < 0.001) of EUB338-probed DAPI cells observed on in situ glass slides, but also fewer gamma -Proteobacteria (12%-21%) and more alpha- Proteobacteria (16%-33%) when compared to direct sand extracts. Hence, investigators of the microbial ecology of even simple sand biofilms must consider theinherent biases from "accepted" methods and seek further independent methods to identify those which may be most accurate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:04:23