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Titolo:
Activity determination of Na+K+-ATPase and Mg++-ATPase enzymes in the gillof Poecilia vivipara (Osteichthyes, Cyprinodontiformes) in different salinities
Autore:
Amaral, MD; Bonecker, ACT; Ortiz, CHD;
Indirizzi:
Univ Fed Rio de Janeiro, Inst Biol, Dept Zool, CCS, BR-21491590 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21491590 BCeiro, Brazil Univ Fed Rio de Janeiro, Inst Quim, Dept Bioquim, BR-21910240 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21910240 BCeiro, Brazil
Titolo Testata:
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
fascicolo: 1, volume: 44, anno: 2001,
pagine: 1 - 6
SICI:
1516-8913(200103)44:1<1:ADONAM>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ONCORHYNCHUS-KISUTCH WALBAUM; OSMOTIC ADAPTATION; SEA-WATER;
Keywords:
Poecilia vivipara; Na+K+ ATPase; Mg++ ATPase; osmoregulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Bonecker, ACT Univ Fed Rio de Janeiro, Inst Biol, Dept Zool, CCS, Bloco A,BR-21491590 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Bloco A Rio De Janeiro Brazil BR-21491590 BC
Citazione:
M.D. Amaral et al., "Activity determination of Na+K+-ATPase and Mg++-ATPase enzymes in the gillof Poecilia vivipara (Osteichthyes, Cyprinodontiformes) in different salinities", BRAZ ARCH B, 44(1), 2001, pp. 1-6

Abstract

This work aimed to know the tolerance mechanisms through the salinity variation by Na+ K+ - ATPase and Mg++- ATPase and enzymes encountered in the gills of Poecilia vivipara. Infield, the presence of this species was observed in salinities of 0 and 28 parts per thousand. In laboratory, these fish were maintained in aquarium with mean salinity of 30 parts per thousand and positive responses were obtained. Some adult specimens, collected in a lagoon of the Coqueiros Beach, were utilized as matrixes. In the experiments the specimens used were those born in the test aquarium. For each salinity studied three replicates were made with three specimens for each one. The alevins were maintained in salinities of 5, 10, 15, 20, 25, 30 and 35 parts per thousand during a month for adaptation. Gills were extracted in appropriate buffer for isolation of plasma membrane and used for specific dosage of the total enzymatic activity of Na+ K+ - ATPase and Mg++ - ATPase. The relation of alevins to their adaptation towards the salinity variation was alsostudied. The activity of the two enzymes showed a different result. The major expression of Na+ K+ - ATPase was observed in 20 parts per thousand (35mu moles Pi.mg protein.h(-1)), the best salinity to cultivate P. vivipara.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:47:52