Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comparison in localization between cystatin C and cathepsin K in osteoclasts and other cells in mouse tibia epiphysis by immunolight and immunoelectron microscopy
Autore:
Yamaza, T; Tsuji, Y; Goto, T; Kido, MA; Nishijma, K; Moroi, R; Akamine, A; Tanaka, T;
Indirizzi:
Kyushu Univ, Dept Endodontol & Operat Dent, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 & Operat Dent, Fukuoka 8128582, Japan Kyushu Univ, Dept Orthodont, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 Dept Orthodont, Fukuoka 8128582, Japan Kyushu Univ, Dept Oral Anat & Cell Biol, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 at & Cell Biol, Fukuoka 8128582, Japan Kyushu Univ, Grad Sch Dent Sci, Dept Fixed Prosthodont, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ed Prosthodont, Fukuoka 8128582, Japan
Titolo Testata:
BONE
fascicolo: 1, volume: 29, anno: 2001,
pagine: 42 - 53
SICI:
8756-3282(200107)29:1<42:CILBCC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTEINE-PROTEINASE-INHIBITOR; AMINO-ACID-SEQUENCE; BONE-RESORPTION; LYSOSOMAL CYSTEINE; MOLECULAR-CLONING; RAT OSTEOCLASTS; IMMUNOCYTOCHEMICAL LOCALIZATION; TISSUE DISTRIBUTION; DISSOLVED CALCIUM; PROTON PUMP;
Keywords:
cystatin C; cathepsin K; osteoclast; tibia epiphysis; immunolight microscopy; electronmicroscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, T Kyushu Univ 61, Grad Sch Dent Sci, Dept Oral Anat & Cell Biol, Fukuoka 8128582, Japan Kyushu Univ 61 Fukuoka Japan 8128582 l, Fukuoka 8128582, Japan
Citazione:
T. Yamaza et al., "Comparison in localization between cystatin C and cathepsin K in osteoclasts and other cells in mouse tibia epiphysis by immunolight and immunoelectron microscopy", BONE, 29(1), 2001, pp. 42-53

Abstract

We compared the distribution of a cysteine proteinase inhibitor, cystatin C, with that of cathepsin K in osteoclasts of the mouse tibia by immunolight and immunoelectron microscopy. Light microscopically, strong immunoreactivity for cystatin C was found extracellularly along the resorption lacuna and intracellularly in the organelles of osteoclasts. In serial sections, various patterns of cystatin C and cathepsin K localization were seen, specifically: (1) some resorption lacuna were positive for both cystatin C and cathepsin K; (2) others were positive for either cystatin C or cathepsin K, but not both; and (3) some lacuna were negative for both. In osteoclasts, the localization of cystatin C was similar to that of cathepsin K. Furthermore, cystatin C immunoreactivity was detected in preosteoclasts and osteoblasts, whereas cathepsin K was seen only in preosteoclasts. Electron microscopically, cystatin C immunoreactive products were found in the rough endoplasmic reticulum (ER), Golgi apparatus, vesicles, granules, and vacuoles of osteoclasts. These cystatin C-positive vesicles had fused or were in the process of fusion with the ampullar vacuoles (extracellular spaces) containing cystatin C-positive, fragmented, fibril-like structures. The extracellular cystatin C was deposited on and between the cytoplasmic processes of ruffled borders, and on and between type I collagen fibrils. In the basolateral region of osteoclasts, cystatin C-positive vesicles and granules also fused with vacuoles that contained cystatin C-positive or negative fibril-like structures. These results indicate that osteoclasts not only synthesize and secrete cathepsin K from the ruffled border into the bone resorption lacunae, but also a cysteine proteinase inhibitor, cystatin C. Therefore, it is suggested that cystatin C regulates the degradation of bone matrix by cathepsin K, both extracellularly and intracellularly. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:49:58