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Titolo:
The effect of 17 beta-estradiol on production of cytokines in cultures of peripheral blood
Autore:
Rogers, A; Eastell, R;
Indirizzi:
Univ Sheffield, Div Clin Sci N, Med Sect, Bone Metab Grp, Sheffield S10 2TN, S Yorkshire, England Univ Sheffield Sheffield S Yorkshire England S10 2TN S Yorkshire, England
Titolo Testata:
BONE
fascicolo: 1, volume: 29, anno: 2001,
pagine: 30 - 34
SICI:
8756-3282(200107)29:1<30:TEO1BO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSTMENOPAUSAL WOMEN; MONONUCLEAR-CELLS; ESTROGEN; MONOCYTES; INVITRO; THERAPY; RELEASE; IL-6;
Keywords:
17 beta-estradiol; postmenopausal women; whole blood; cultures; cytokines; in vitro;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Eastell, R No Gen Hosp, Ctr Clin Sci, Bone Metab Grp, Herries Rd, Sheffield S5 7AU, SYorkshire, England No Gen Hosp Herries Rd Sheffield S Yorkshire England S5 7AU and
Citazione:
A. Rogers e R. Eastell, "The effect of 17 beta-estradiol on production of cytokines in cultures of peripheral blood", BONE, 29(1), 2001, pp. 30-34

Abstract

Estrogen's action on bone may be mediated by cytokines produced by monocytes. We have reported a decreased ratio of interleuldn-1 beta (IL-1 beta) tointerleukin-1 receptor antagonist (IL-1ra) produced by whole blood cultures in vivo in women taking hormone replacement therapy (HRT). Also, one study has shown an effect of estradiol on tumor necrosis factor-alpha (TNT-alpha) secretion by separated monocytes in vitro. The aim of this study was to evaluate the effect of estrogen in vitro on the secretion of cytokines using whole blood cultures. Subjects consisted of 12 healthy postmenopausal women, ages 57-69 years, 4-20 years since menopause. Cytokines IL-1 beta, interleukin-1 alpha (IL-1 alpha), IL-1ra, interleukin-6 (IL-6), TNF-alpha, and granulocyte macrophagecolony stimulating factor (GM-CSF) were measured in unstimulated and in stimulated (500 ng/mL lipopolysaccharide [LPS]) whole blood cultures treated with 17 beta -estradiol (E-2) at concentrations of 10(-12)-10(-6) mol/L. We found significant decreases in the spontaneous secretion of IL-6, TNF-alpha, EL-1ra, IL-1 beta, and ratio of IL-1 beta /IL-1ra compared with control, at physiological concentrations of E-2. The action ofE-2 was blocked by the use of the antiestrogen ICI 182780 in coculture. A decrease in cytokine secretion was not observed when the inactive form of estrogen, 17 alpha -estradiol, was used in place of 17 beta -estradiol. GM-CSF and IL-1 alpha were not detectable in unstimulated cultures. Cytokine levels measured in stimulated cultures were not attenuated by treatment with E-2. We conclude that E-2 inhibits the spontaneous secretion of cytokines measured in whole blood cultures at physiological concentrations, and that the powerful stimulatory effect of LPS prevents any significant inhibition by E-2 in stimulated cultures. (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:09:54