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Titolo:
Cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway from a terpenoid-antibiotic-producing Streptomyces strain
Autore:
Hamano, Y; Dairi, T; Yamamoto, M; Kawasaki, T; Kaneda, K; Kuzuyama, T; Itoh, N; Seto, H;
Indirizzi:
Toyama Prefectural Univ, Biotechnol Res Ctr, Toyama 9390398, Japan Toyama Prefectural Univ Toyama Japan 9390398 Ctr, Toyama 9390398, Japan Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan Univ Tokyo Tokyo Japan 1130032 r Biosci, Bunkyo Ku, Tokyo 1130032, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 7, volume: 65, anno: 2001,
pagine: 1627 - 1635
SICI:
0916-8451(200107)65:7<1627:COAGCE>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME-A REDUCTASE; NONMEVALONATE PATHWAY; 4-(CYTIDINE 5'-DIPHOSPHO)-2-C-METHYL-D-ERYTHRITOL; SACCHAROPOLYSPORA-ERYTHRAEA; ISOPRENOID BIOSYNTHESIS; ISOPENTENYL DIPHOSPHATE; 5-PHOSPHATE; PROTEIN; KINASE; 4-DIPHOSPHOCYTIDYL-2C-METHYL-D-ERYTHRITOL;
Keywords:
mevalonate pathway; gene cloning; Streptomyces; terpenoid; terpentecin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Dairi, T Toyama Prefectural Univ, Biotechnol Res Ctr, Toyama 9390398, Japan Toyama Prefectural Univ Toyama Japan 9390398 ama 9390398, Japan
Citazione:
Y. Hamano et al., "Cloning of a gene cluster encoding enzymes responsible for the mevalonate pathway from a terpenoid-antibiotic-producing Streptomyces strain", BIOS BIOT B, 65(7), 2001, pp. 1627-1635

Abstract

A gene cluster encoding enzymes responsible for the mevalonate pathway wasisolated from Streptomyces griseolosporeus strain MF730-N6, a terpenoid-antibiotic terpentecin producer, by searching a flanking region of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase gene, which had been previously isolated by complementation. By DNA sequencing of an 8.9-kb BamHI fragment, 7 genes encoding geranylgeranyl diphosphate synthase (GGDPS), mevalonate kinase (MK), mevalonate diphosphate decarboxylase (MDPD), phosphomevalonate kinase (PMK), isopentenyl diphosphate (IPP) isomerase, HMG-CoA reductase, and HMG-CoA synthase were suggested to exist in that order. Heterologous expression of these genes in E. coli and Streptomyces lividans, both of which have only the nonmevalonate pathways, suggested that the genes forthe mevalonate pathway were included in the cloned DNA fragment. The GGDPS, MK, MDPD, PMK, IPP isomerase, and HMG-CoA synthase were expressed in E. coli. Among them, the recombinant GGDPS, MK, and IPP isomerase were confirmed to have the expected activities. This is the first report, to the best ofour knowledge, about eubacterial MK with direct evidence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 07:03:52