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Titolo:
Is your ribozyme design really correct?: A proposal of simple single turnover competition assay to evaluate ribozymes
Autore:
Tanaka, T; Inui, O; Dohi, N; Okada, N; Okada, H; Kikuchi, Y;
Indirizzi:
Toyohashi Univ Technol, Dept Ecol Engn, Div Biosci & Biotechnol, Aichi 4418580, Japan Toyohashi Univ Technol Aichi Japan 4418580 technol, Aichi 4418580, Japan Nagoya City Univ, Sch Med, Dept Mol Biol, Mizuho Ku, Nagoya, Aichi 4678601, Japan Nagoya City Univ Nagoya Aichi Japan 4678601 Nagoya, Aichi 4678601, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 7, volume: 65, anno: 2001,
pagine: 1636 - 1644
SICI:
0916-8451(200107)65:7<1636:IYRDRC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLEAVING DNA ENZYME; HAIRPIN RIBOZYME; HAMMERHEAD RIBOZYME; MESSENGER-RNA; CLEAVAGE; SEQUENCE; SPECIFICITIES; TRANSCRIPTION; INHIBITION; SUBSTRATE;
Keywords:
hammerhead ribozyme; hairpin ribozyme; DNA-enzyme; 5I2 antigen mRNA; competition assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, T Toyohashi Univ Technol, Dept Ecol Engn, Div Biosci & Biotechnol,Tempaku Cho, Aichi 4418580, Japan Toyohashi Univ Technol Tempaku Cho Aichi Japan 4418580 0, Japan
Citazione:
T. Tanaka et al., "Is your ribozyme design really correct?: A proposal of simple single turnover competition assay to evaluate ribozymes", BIOS BIOT B, 65(7), 2001, pp. 1636-1644

Abstract

Today, many nucleic acid enzymes are used in gene therapy and gene regulations. However, no simple assay methods to evaluate enzymatic activities, with which we judge the enzyme design, have been reported. Here, we propose anew simple competition assay for nucleic acid enzymes of different types to evaluate the cleaving efficiency of a target RNA molecule, of which the recognition sites are different but overlapped. Two nucleic acid enzymes were added to one tube to make a competition of these two enzymes for one substrate. The assay was used on two ribozymes, hammerhead ribozyme and hairpinribozyme, and a DNA-enzyme. We found that this assay method is capable of application to those enzymes, as a powerful tool for the selection and designing of RNA-cleaving enzymes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:54:50