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Titolo:
Azurin involved in alcohol oxidation system in Pseudomonas putida HK5: Expression analysis and gene cloning
Autore:
Toyama, H; Aoki, N; Matsushita, K; Adachi, O;
Indirizzi:
Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 7538515, Japan Yamaguchi Univ Yamaguchi Japan 7538515 ol Chem, Yamaguchi 7538515, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 7, volume: 65, anno: 2001,
pagine: 1617 - 1626
SICI:
0916-8451(200107)65:7<1617:AIIAOS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-TRANSPORT CHAIN; BLUE COPPER PROTEIN; 2 DISTINCT AZURINS; ESCHERICHIA-COLI; GLUCONOBACTER-SUBOXYDANS; ALCALIGENES-FAECALIS; NITRITE REDUCTASE; AERUGINOSA; FNR; DEHYDROGENASES;
Keywords:
azurin; quinoprotein; pyrroloquinoline quinone; PQQ; alcohol dehydrogenase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Toyama, H Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 7538515, Japan Yamaguchi Univ Yamaguchi Japan 7538515 amaguchi 7538515, Japan
Citazione:
H. Toyama et al., "Azurin involved in alcohol oxidation system in Pseudomonas putida HK5: Expression analysis and gene cloning", BIOS BIOT B, 65(7), 2001, pp. 1617-1626

Abstract

Expression of azurin in Pseudomonas Putida HK5 was examined by immunoblot analysis. Similar amounts of azurin were found in the cells grown into the stationary phase on any carbon sources, including LB medium without alcohol, where no quinoprotein alcohol dehydrogenases appeared. In the early exponential phase, the highest amount of azurin was found in the cells grown on 1-butanol, but here was none in the case of LB medium, suggesting that expression of azurin is cooperative with that of the alcohol oxidase system, especially the system including quinohemoprotein alcohol dehydrogenase IIB. The azurin gene (azu) was cloned and sequenced. azu is monocistronic, and in its promoter region, FNR-binding consensus sequence was found. However, its relative position suggests different transcriptional regulation from that in azu of P. aeruginosa. The molecular weight of the mature protein without copper ion calculated from the amino acid sequence was consistent with the value of the purified azurin measured by mass spectrometry.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:35:10