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Titolo:
In vivo and in vitro analyses of the AmyR binding site of the Aspergillus nidulans agdA promoter; Requirement of the CGG direct repeat for induction and high affinity binding of AmyR
Autore:
Tani, S; Itoh, T; Kato, M; Kobayashi, T; Tsukagoshi, N;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Dept Biol Mech & Funct, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 7, volume: 65, anno: 2001,
pagine: 1568 - 1574
SICI:
0916-8451(200107)65:7<1568:IVAIVA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAKA-AMYLASE-A; DNA-BINDING; NUCLEOTIDE-SEQUENCE; TRANSCRIPTIONAL ACTIVATOR; BINUCLEAR CLUSTER; ALPHA-GLUCOSIDASE; ORYZAE; GENE; EXPRESSION; ELEMENTS;
Keywords:
AmyR; Aspergillus nidulans; amylase induction; DNA binding; transcriptional activator;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, T Nagoya Univ, Grad Sch Bioagr Sci, Dept Biol Mech & Funct, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 Aichi 4648601, Japan
Citazione:
S. Tani et al., "In vivo and in vitro analyses of the AmyR binding site of the Aspergillus nidulans agdA promoter; Requirement of the CGG direct repeat for induction and high affinity binding of AmyR", BIOS BIOT B, 65(7), 2001, pp. 1568-1574

Abstract

The alpha -glucosidase gene (agdA) of Aspergillus nidulans has a single CGGN(8)CGG type AmyR binding site in its promoter region. The binding site isfunctional in vivo as a cis-element responsible for induction by starch, and mutational studies indicated that both the CGG triplets are required forhigh-level induction. A part of AmyR (residues 1-411; AmyR(1-411)), which was produced as a MalEfusion protein in E. coli, bound to the CGGN(8)CGG site of the agdA promoter. DNA binding profiles to the mutant binding sites that lacked both or either one of the CGG triplets suggested that AmyR(1-411) can bind to a single CGG triplet site with low affinity and that two AmyR molecules cooperatively bind to the CGG direct repeat.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 11:16:26