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Titolo:
Oestrogenic activity of CPRG (chlorophenol red-beta-D-galactopyranoside), a beta-galactosidase substrate commonly used in recombinant yeast oestrogenic assays
Autore:
Vanderperren, E; Demare, W; Blust, R; Cooreman, K; Bossier, P;
Indirizzi:
Agr Res Ctr Ghent, Dept Sea Fisheries, Minist Small Enterprises Traders & Agr, B-8400 Oostende, Belgium Agr Res Ctr Ghent Oostende Belgium B-8400 Agr, B-8400 Oostende, Belgium Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium Univ Antwerp Antwerp Belgium B-2020 , Dept Biol, B-2020 Antwerp, Belgium
Titolo Testata:
BIOMARKERS
fascicolo: 5, volume: 6, anno: 2001,
pagine: 375 - 380
SICI:
1354-750X(200109)6:5<375:OAOC(R>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROGEN-RECEPTOR; CHEMICALS; CELLS;
Keywords:
oestrogenic activity; yeast assay; chlorophenol red-beta-D-galactopyranoside (CPRG);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Vanderperren, E Agr Res Ctr Ghent, Dept Sea Fisheries, Minist Small Enterprises Traders & Agr, Ankerstr 1, B-8400 Oostende, Belgium Agr Res Ctr GhentAnkerstr 1 Oostende Belgium B-8400 gium
Citazione:
E. Vanderperren et al., "Oestrogenic activity of CPRG (chlorophenol red-beta-D-galactopyranoside), a beta-galactosidase substrate commonly used in recombinant yeast oestrogenic assays", BIOMARKERS, 6(5), 2001, pp. 375-380

Abstract

The finding that a variety of chemicals display oestrogenic activity has resulted in the development of in vitro and in vivo assays to assess oestrogenic activity. One such assay, the yeast oestrogen assay (YES) makes use ofrecombinant yeast cells that harbour an oestrogen receptor expression cassette and a reporter construct, coding for beta -galactosidase. The induction mechanism starts with the binding of oestrogenic compounds to the oestrogen receptor. This complex activates the production of beta -galactosidase. The beta -galactosidase activity is thus a measure of the oestrogenic activity of chemical compounds. In the YES assay, the beta -galactosidase activity may be quantified with the chromogenic substrate chlorophenol red-beta -D-galactopyranoside (CPRG). In the present study it is reported that CPRG or its beta -galactosidase degradation product chlorophenol red act in the YES as an oestrogenic compound itself. The implications of this finding are described. It is especially argued that chlorophenol red production after prolonged incubation of the assay might be misinterpreted as an oestrogenic effect of the test compound.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:43:01