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Titolo:
TRANSCRIPTIONAL REGULATION OF PILC2 IN NEISSERIA-GONORRHOEAE - RESPONSE TO OXYGEN AVAILABILITY AND EVIDENCE FOR GROWTH-PHASE REGULATION IN ESCHERICHIA-COLI
Autore:
MELLIES J; RUDEL T; MEYER TF;
Indirizzi:
MAX PLANCK INST BIOL,INFEKT BIOL ABT,SPEMANNSTR 34 D-72076 TUBINGEN GERMANY MAX PLANCK INST BIOL,INFEKT BIOL ABT D-72076 TUBINGEN GERMANY MAX PLANCK INST INFEKT BIOL D-10117 BERLIN GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 3, volume: 255, anno: 1997,
pagine: 285 - 293
SICI:
0026-8925(1997)255:3<285:TROPIN>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEIN; HUMAN EPITHELIAL-CELLS; SALMONELLA-TYPHIMURIUM; ANAEROBIC GROWTH; YERSINIA-ENTEROCOLITICA; GENE-EXPRESSION; ADHERENCE; IDENTIFICATION; REPRESSION; PROMOTERS;
Keywords:
GONOCOCCI; PILLI; TRANSCRIPTION; ANAEROBIOSIS; GROWTH PHASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
J. Mellies et al., "TRANSCRIPTIONAL REGULATION OF PILC2 IN NEISSERIA-GONORRHOEAE - RESPONSE TO OXYGEN AVAILABILITY AND EVIDENCE FOR GROWTH-PHASE REGULATION IN ESCHERICHIA-COLI", MGG. Molecular & general genetics, 255(3), 1997, pp. 285-293

Abstract

The type-4 pilus of Neisseriu gonorrhoeae is a dominant surface antigen which facilitates adhesion to host target cells, an essential eventin gonococcal infection. pilC2 encodes a 110-kDa protein involved in pilus assembly, pilus-mediated adherence to human epithelial cells in culture and natural competence for DNA transformation. Luciferase activity directed from a chromosomal pilC2::luxAB transcriptional fusion was reduced approximately 4-fold when cells were grown anaerobically. We observed a concomitant reduction in gonococcal piliation by electronmicroscopy and a reduction in the ability to adhere to ME-180 human epithelial cells when bacteria were grown in the absence of oxygen. Furthermore, we present evidence for growth-phase regulation of the gonococcal pilC2 gene in Escherichia coli, and show that all sequences necessary for growth-phase regulation are contained on a 121-bp pilC2 fragment. Expression from the minimal pilC2 fragment fused to lacZ in single-copy in E. coli was induced 2-fold when cells entered stationary phase. Surprisingly, induction does not require rpoS, the gene, which encodes the starvation-induced sima factor RpoS. In summary, we have demonstrated that pilC2 is both positively and negatively regulated at the level of transcription. This regulation is most probably relevant tophysiological conditions within the human host which influence gonococcal infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 22:44:06