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Titolo:
Very-long-chain fatty acid-containing phospholipids accumulate in fatty acid synthase temperature-sensitive mutant strains of the fission yeast Schizosaccharomyces pombe fas2/lsd1
Autore:
Yokoyama, K; Saitoh, S; Ishida, M; Yamakawa, Y; Nakamura, K; Inoue, K; Taguchi, R; Tokumura, A; Nishijima, M; Yanagida, M; Setaka, M;
Indirizzi:
Teikyo Univ, Fac Pharmaceut Sci, Kanagawa 1990195, Japan Teikyo Univ Kanagawa Japan 1990195 armaceut Sci, Kanagawa 1990195, Japan Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Grad Sch Biostudies, Kyoto 6068502, Japan Nagoya City Univ, Fac Pharmaceut Sci, Mizuho Ku, Aichi 4670027, Japan Nagoya City Univ Aichi Japan 4670027 ci, Mizuho Ku, Aichi 4670027, Japan Natl Inst Infect Dis, Dept Biochem & Cell Biol, Shinjuku Ku, Tokyo 1628640, Japan Natl Inst Infect Dis Tokyo Japan 1628640 injuku Ku, Tokyo 1628640, Japan Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan Univ Tokushima Tokushima Japan 7708505 eut Sci, Tokushima 7708505, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 3, volume: 1532, anno: 2001,
pagine: 223 - 233
SICI:
1388-1981(20010629)1532:3<223:VFAPAI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE; PEROXISOMAL DISORDERS; BINDING-SITES; GENE; SYNTHETASE; SEQUENCE; PROTEIN; IDENTIFICATION; SPHINGOLIPIDS; SPHINGOMYELIN;
Keywords:
very-long-chain fatty acid; fatty acid synthase; melissate; triacontanate; two-dimensional thin-layer chromatography; electrospray/mass spectrometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Yokoyama, K Univ Tennessee, Hlth Sci Ctr, Dept Physiol, 894 Union Ave, Memphis, TN 38163 USA Univ Tennessee 894 Union Ave Memphis TN USA 38163 TN 38163 USA
Citazione:
K. Yokoyama et al., "Very-long-chain fatty acid-containing phospholipids accumulate in fatty acid synthase temperature-sensitive mutant strains of the fission yeast Schizosaccharomyces pombe fas2/lsd1", BBA-MOL C B, 1532(3), 2001, pp. 223-233

Abstract

Fission yeast Isd1 strains show aberrant mitosis with a Isd phenotype, large and small daughter nuclei, and a very thick septum, the phenotypic expression being temperature-sensitive. The Isd1(+) gene is the homologue of thebudding yeast FAS2 gene encoding the fatty acid synthase alpha -subunit asreported previously (S. Saitoh, K. Takahashi, K. Nabeshima, Y. Yamashita, Y. Nakaseko, A. Hirata, M. Yanagida, J. Cell Biol. 134 (1996) 949-961). In this paper, Isd1 is considered to represent fas2. Here, three fas2 strains were investigated and found to have missense point mutations at different sites in the,gene encoding the alpha -subunit of fatty acid synthase. The mutation affected only slightly the enzymatic activities monitored in vitro. Unexpectedly, abnormal phospholipids, phosphatidyleholine and phosphatidylethanolamine, both of which contain a very-long-chain fatty acyl residue (1-melissoyl-2-oleolyl-sn-glycero-3-phosphocholine and 1-melissoyl-2-oleolyl-sn-glycero-3-phosphoethanolamine), accumulated in fas2 strains in a temperature-sensitive manner. Rescue of the fas2 strains by addition of palmitate to the medium at restrictive temperature was accompanied by disappearance ofthese abnormal phospholipids. Accumulation of these lipids in membranes may cause alteration of various cellular functions. (C) 2001 Published by Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 13:16:20