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Titolo:
Significant improvement of taxane production in suspension cultures of Taxus chinensis by combining elicitation with sucrose feed
Autore:
Dong, HD; Zhong, JJ;
Indirizzi:
E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China E China Univ Sci & Technol Shanghai Peoples R China 200237 oples R China
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 2, volume: 8, anno: 2001,
pagine: 145 - 150
SICI:
1369-703X(200109)8:2<145:SIOTPI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYL JASMONATE; PACLITAXEL PRODUCTION; CELL-CULTURES; TAXOL; BREVIFOLIA; CUSPIDATA; SYNTHASE; BACCATA;
Keywords:
Taxus chinensis; taxuyunnanine C; elicitation; sucrose feed; combination strategy; plant cell culture; bioprocess engineering;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Zhong, JJ E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd,Shanghai 200237, Peoples R China E China Univ Sci & Technol 130 Meilong Rd Shanghai Peoples R China 200237
Citazione:
H.D. Dong e J.J. Zhong, "Significant improvement of taxane production in suspension cultures of Taxus chinensis by combining elicitation with sucrose feed", BIOCH ENG J, 8(2), 2001, pp. 145-150

Abstract

The effects of elicitation and its combination with sucrose feed on the cell growth and accumulation of taxane diterpene - taxuyunnanine C (Tc) in suspension cultures of Taxus chinensis were investigated in shake flasks. TheTc accumulation was enhanced by elicitation of methyl jasmonate (MJA) or dihydro-methyl jasmonate (HMJA), and addition of 100 ELM MJA or HMJA at day 7 could increase the Tc content to 21.9 +/- 0.8 and 15.8 +/- 1.8 mg (g DW)(-1), respectively, without any inhibitory effects on the cell growth. The activity of a key enzyme - taxadiene synthase was greatly increased after elicitation. The Tc production was further enhanced by combining elicitation with sucrose feed, and a very high production titer of 527 +/- 17 mg l(-1) was achieved on day 15 when elicitation (using 100 muM MJA) was combined with sucrose feed (20 g l(-1)) on day 7. The taxane production titer obtainedin this work was the highest as ever reported. (C) 2001 Elsevier Science B. V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:29:24