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Titolo:
Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture
Autore:
Yamada, K; Kamihira, M; Iijima, S;
Indirizzi:
Nagoya Univ, Dept Biotechnol, Grad Sch Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan Nagoya Univ Nagoya Aichi Japan 4648603 a Ku, Nagoya, Aichi 4648603, Japan
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 2, volume: 8, anno: 2001,
pagine: 135 - 143
SICI:
1369-703X(200109)8:2<135:SOLCCM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT-RAT HEPATOCYTES; HIGH ALBUMIN PRODUCTION; MULTICELLULAR SPHEROIDS; EXTRACELLULAR-MATRIX; BIOARTIFICIAL LIVER; MONOLAYER-CULTURE; IN-VITRO; MAINTENANCE; EXPRESSION; PROLIFERATION;
Keywords:
artificial matrix; bioartificial liver; cytochrome P-450; hepatocyte; hetero-spheroid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kamihira, M Nagoya Univ, Dept Biotechnol, Grad Sch Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan Nagoya Univ Nagoya Aichi Japan 4648603 , Aichi 4648603, Japan
Citazione:
K. Yamada et al., "Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture", BIOCH ENG J, 8(2), 2001, pp. 135-143

Abstract

We previously described the mass preparation of hepatocyte spheroids in a cell suspension. Addition of a synthetic polymer, Eudragit S100 (a co-polymer of methacrylic acid and methylmethacrylate), to the culture medium as anartificial matrix has been found to promote cell aggregation and induce spheroid formation. The present study examines co-cultures of hepatocytes andliver non-parenchymal cells to further enhance the stable expression of liver functions. Hetero-spheroids consisting of hepatocytes and non-parenchymal cells were also formed by adding the polymer to the culture medium. The expression levels of liver functions depended on the mixing ratio of the cells. The values of albumin secretion, ammonia removal and urea synthesis were the highest when equal number of hepatocytes and non-parenchymal cells were mixed. The hetero-spheroids maintained a higher level of albumin secretion for over 4 weeks compared with co-cultured cells without the polymer and spheroids consisting of hepatocytes solely. Other liver functions such asammonia removal and cytochrome P-450 activity in hetero-spheroid cultures were also maintained at a high level throughout the 4-week culture period. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 03:37:31