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Titolo:
Evaluation of growth property of red beet hairy roots depending on condition of inocula and its application to culture control with fuzzy logic theory
Autore:
Takahashi, Y; Hitaka, Y; Kino-oka, M; Taya, M; Tone, S;
Indirizzi:
Osaka Univ, Dept Sci & Chem Engn, Toyonaka, Osaka 5608531, Japan Osaka Univ Toyonaka Osaka Japan 5608531 n, Toyonaka, Osaka 5608531, Japan
Titolo Testata:
BIOCHEMICAL ENGINEERING JOURNAL
fascicolo: 2, volume: 8, anno: 2001,
pagine: 121 - 127
SICI:
1369-703X(200109)8:2<121:EOGPOR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-CELL; ZEA-MAYS; ACCLIMATION; TIPS; ENHANCEMENT; BIOREACTOR; STARVATION; STRESS; FLOW;
Keywords:
plant cell culture; red beet hairy roots; shear stress effect; fuzzy control; lag phase; inoculum condition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Taya, M Osaka Univ, Dept Sci & Chem Engn, 1-3 Machikaneyama Cho, Toyonaka,Osaka 5608531, Japan Osaka Univ 1-3 Machikaneyama Cho Toyonaka Osaka Japan5608531 apan
Citazione:
Y. Takahashi et al., "Evaluation of growth property of red beet hairy roots depending on condition of inocula and its application to culture control with fuzzy logic theory", BIOCH ENG J, 8(2), 2001, pp. 121-127

Abstract

The influence of period of preculture on growth was examined for the development of control system suitable to the culture of red beet hairy roots. The prolongation of preculture caused the decrease in viability of root tips, resulting in the extension of lag phase during the following culture. Thenumber of lateral roots emerging from the inoculated hairy roots could be used as a parameter for the identification of the culture phases. A bioreactor system including a solid-culture unit to monitor the variation in the number of growing points (GPs) was constructed, and the fuzzy inferencing was employed for the control of shear stress in the culture, based on the membership functions with state variables concerning the growth indexes in terms of the number of GPs and concentration of cell mass. The successful performances were achieved by means of appropriate recognition of culture phases in the cultures of hairy roots with different levels of tip viability. Itwas verified that this control system could be available for the culture with variation of inoculum states by employing the state variable based on the number of elongating tip meristems of the hairy roots. (C) 2001 ElsevierScience B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 11:02:01