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Titolo:
Association of MUC-1 and PSGL-1 with low-density microdomain in T-lymphocytes: A preliminary note
Autore:
Handa, K; Jacobs, F; Longenecker, BM; Hakomori, S;
Indirizzi:
Pacific NW Res Inst, Div Biomembrane, Seattle, WA 98122 USA Pacific NW ResInst Seattle WA USA 98122 omembrane, Seattle, WA 98122 USA Univ Washington, Dept Pathobiol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Pathobiol, Seattle, WA 98195 USA Univ Washington, Dept Microbiol, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 ept Microbiol, Seattle, WA 98195 USA Biomira Inc, Edmonton, AB T6N 1H1, Canada Biomira Inc Edmonton AB Canada T6N 1H1 Inc, Edmonton, AB T6N 1H1, Canada
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 3, volume: 285, anno: 2001,
pagine: 788 - 794
SICI:
0006-291X(20010720)285:3<788:AOMAPW>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SELECTIN-DEPENDENT ADHESION; MELANOMA B16 CELLS; P-SELECTIN; SIGNAL-TRANSDUCTION; MOLECULAR-CLONING; MEMBRANE; MUCIN; SIALOSYL-LE(X); GLYCOSYLATION; EXPRESSION;
Keywords:
MUC-1; PSGL-1; glycosylation-enriched microdomain; Fyn; Yes; lck(56); methyl beta-cyclodextrin; signal transducer; T-lymphocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Handa, K Pacific NW Res Inst, Div Biomembrane, 720 Broadway, Seattle, WA 98122 USA Pacific NW Res Inst 720 Broadway Seattle WA USA 98122 A 98122 USA
Citazione:
K. Handa et al., "Association of MUC-1 and PSGL-1 with low-density microdomain in T-lymphocytes: A preliminary note", BIOC BIOP R, 285(3), 2001, pp. 788-794

Abstract

Two mucin-type glycoproteins, MUC-1 and P-selectin glycoprotein ligand-1 (PSGL-1), and glycosphingolipids (GSLs), expressed in human T-cell line HUT78, were highly enriched in low-density buoyant fraction (termed "GEM"), together with CD45, Yes, Fyn, and Ick(56). Enrichment of MUC-1, PSGL-1 and GSLs, together with these signal transducer molecules in low-density membrane fraction was observable when fraction was prepared from cells either in nonionic detergent Brij 58 or in hypertonic alkaline conditions (500 mM Na2CO3). On pretreatment of cells with cholesterol-binding reagent methyl beta -cyclodextrin, levels of MUC-1 and PSGL-1 together with the above signal transducers in GEM was greatly reduced, and they were translocated into high-density membrane fraction. Similar association of lck(58), Yes, Fyn, and cSrctogether with MUC-1 was also found in GEM fraction of mouse T-cell lymphoma EL4 cells expressing MUC-1 through transfection of its gene. These findings indicate the presence of another glycosyl cluster ("glycocluster"), in addition to the previously well-established GSL cluster organized with signal transducer molecules in GEM fraction, and its possible functional role inT-cells. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:53:39