Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reversible integration of the dominant negative retinoid receptor gene forex vivo expansion of hematopoietic stem/progenitor cells
Autore:
Muramatsu, M; Hanazono, Y; Ogasawara, Y; Okada, T; Mizukami, H; Kume, A; Mizoguchi, H; Ozawa, K;
Indirizzi:
Jichi Med Sch, Ctr Mol Med, Div Genet Therapeut, Kawachi, Tochigi 3290498,Japan Jichi Med Sch Kawachi Tochigi Japan 3290498 awachi, Tochigi 3290498,Japan Jichi Med Sch, Dept Med, Div Hematol, Kawachi, Tochigi 3290498, Japan Jichi Med Sch Kawachi Tochigi Japan 3290498 wachi, Tochigi 3290498, Japan Tokyo Womens Med Univ, Sch Med, Dept Hematol, Tokyo, Japan Tokyo Womens Med Univ Tokyo Japan , Sch Med, Dept Hematol, Tokyo, Japan Jikei Univ, Sch Med, Dept Hematol & Oncol, Tokyo, Japan Jikei Univ TokyoJapan niv, Sch Med, Dept Hematol & Oncol, Tokyo, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 285, anno: 2001,
pagine: 891 - 896
SICI:
0006-291X(20010727)285:4<891:RIOTDN>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; ACID RECEPTOR; CRE RECOMBINASE; ADENOVIRUS VECTOR; MAMMALIAN-CELLS; STEM-CELLS; DIFFERENTIATION; EXPRESSION; IMMORTALIZATION; PROGENITORS;
Keywords:
ex vivo expansion; hematopoietic stem cell; retrovirus vector; dominant negative retinoic acid receptor; Cre recombinase; loxP; reversible integration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ozawa, K Jichi Med Sch, Ctr Mol Med, Div Genet Therapeut, 3311-1 Yakushiji, Kawachi, Tochigi 3290498, Japan Jichi Med Sch 3311-1 Yakushiji Kawachi Tochigi Japan 3290498 apan
Citazione:
M. Muramatsu et al., "Reversible integration of the dominant negative retinoid receptor gene forex vivo expansion of hematopoietic stem/progenitor cells", BIOC BIOP R, 285(4), 2001, pp. 891-896

Abstract

Since hematopoietic stem cells (HSCs) differentiate readily ex vivo resulting in the loss of self-renewal and engraftment abilities, the transient block of differentiation is essential to maintain those abilities during their ex vivo expansion culture. To this end, we developed a method of reversible integration of the dominant negative retinoic acid receptor (DN-RAR) gene, a differentiation-blocking gene, into cells utilizing the Cre/loxP-dependent gene recombination system. The murine immature hematopoietic 32D cellsdifferentiate into mature neutrophils upon G-CSF treatment. However, 32D cells transduced with a retroviral vector expressing the DN-RAR gene put between two loxP sites continued to proliferate without showing differentiation even in the presence of G-CSF. After the cells were fully amplified, the cells were transduced with the Cre recombinase gene. The cells then restored the ability to differentiate into mature neutrophils upon G-CSF treatment. PCR analysis showed that the DN-RAR gene was efficiently removed from thegenome by introduction of the Cre gene. This system may eventually be applicable to the ex vivo expansion of HSCs. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/05/20 alle ore 13:18:21