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Titolo:
Functional beta(2)-adrenergic receptors are present in nonstrial tissues of the lateral wall in the gerbil cochlea
Autore:
Schimanski, S; Scofield, MA; Wangemann, P;
Indirizzi:
Kansas State Univ, Cell Physiol Lab, Dept Anat & Physiol, Manhattan, KS 66506 USA Kansas State Univ Manhattan KS USA 66506 Physiol, Manhattan, KS 66506 USA Creighton Univ, Dept Pharmacol, Mol Pharmacol Lab, Omaha, NE 68178 USA Creighton Univ Omaha NE USA 68178 Mol Pharmacol Lab, Omaha, NE 68178 USA
Titolo Testata:
AUDIOLOGY AND NEURO-OTOLOGY
fascicolo: 3, volume: 6, anno: 2001,
pagine: 124 - 131
SICI:
1420-3030(200105/06)6:3<124:FBRAPI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENYLYL-CYCLASE ISOFORMS; STRIAL MARGINAL CELLS; GUINEA-PIG; K+ SECRETION; INNER-EAR; BETA(1)-ADRENERGIC RECEPTORS; ENDOLYMPHATIC HYDROPS; VASOPRESSIN RECEPTORS; CHOLERA-TOXIN; LOCALIZATION;
Keywords:
receptor, beta-adrenergic; antagonists, beta-adrenergic; cochlea; spiral ligament; CGP 20712A; ICI 118551;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Wangemann, P Kansas State Univ, Cell Physiol Lab, Dept Anat & Physiol, 1600 Dension Ave, Manhattan, KS 66506 USA Kansas State Univ 1600 Dension Ave Manhattan KS USA 66506 USA
Citazione:
S. Schimanski et al., "Functional beta(2)-adrenergic receptors are present in nonstrial tissues of the lateral wall in the gerbil cochlea", AUDIOL NEUR, 6(3), 2001, pp. 124-131

Abstract

Recently, we have demonstrated that functional beta (1)-adrenergic receptors are the dominant beta -adrenergic receptor subtype in the stria, vascularis and that beta (1)-adrenergic receptors stimulate K+ secretion in strialmarginal cells. The goal of the present study was to determine whether nonstrial tissues in the cochlear lateral wall contain beta -adrenergic receptors and if so which subtype is present. Pharmacological tools were used to identify receptors in functional studies where CAMP production was measured. Further, receptors were identified as transcripts by cloning and sequencing of reverse-transcriptase polymerase chain reaction (RT-PCR) products. Experiments were performed on gerbil nonstrial lateral wall tissues. Tissues stimulated with 10(-5) M isoproterenol produced 0.42 +/- 0.22 pmol CAMP perear within 12 min (n = 14). The selective beta -adrenergic receptor agonist isoproterenol stimulated CAMP production with an EC50 of (2 +/- 3) x 10(-7) M (n = 7). Isoproterenol-stimulated CAMP production was inhibited by thebeta (2)-adrenergic receptor antagonist ICI 118551 with an IC50 of (7 7) x10(-6) M, which corresponds to an affinity constant of 1 x 10(-7) M(pK(DB)=6.89 +/- 0.23, n = 3). Isoproterenol-stimulated CAMP production was not inhibited by the highly selective Pl-adrenergic receptor antagonist CGP 20712A. The IC50 and the affinity constant for CGP 20712A were estimated to be >3 x 10(-4) and >6 x 10(-6) Mr respectively. RT-PCR of total RNA isolated from nonstrial lateral wall tissues using primers specific for beta (1)-, beta (2)- and beta (3)-adrenergic receptors revealed products of the predictedsizes for the beta (1)- and beta (2)- but not for the beta (3)-subtype. Sequence analysis confirmed that amplified cDNA fragments encoded gene-specific nucleotide sequences. These results demonstrate that nonstrial lateral wall tissues contain transcripts for beta (1)- and beta (2)- but not for beta (3)-adrenergic receptors and that the beta (2)-adrenergic receptor is thedominant functional receptor subtype. The cellular localization and function of the beta (2)-adrenergic receptors remains to be determined. Copyright(C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/02/20 alle ore 20:49:16