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Titolo:
Overexpression of copper/zinc-superoxide dismutase protects from kanamycin-induced hearing loss
Autore:
Sha, SH; Zajic, G; Epstein, CJ; Schacht, J;
Indirizzi:
Univ Michigan, Kresge Hearing Res Inst, Dept Otolaryngol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Otolaryngol, Ann Arbor, MI 48109 USA Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
AUDIOLOGY AND NEURO-OTOLOGY
fascicolo: 3, volume: 6, anno: 2001,
pagine: 117 - 123
SICI:
1420-3030(200105/06)6:3<117:OOCDPF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PIG IN-VIVO; FOCAL CEREBRAL-ISCHEMIA; FREE-RADICAL FORMATION; TRANSGENIC MICE; GENTAMICIN OTOTOXICITY; NITRIC-OXIDE; IRON CHELATORS; AMINOGLYCOSIDE ANTIBIOTICS; NEUROTOXICITY; TOXICITY;
Keywords:
aminoglycosides; ototoxicity; protection; reactive oxygen species; superoxide dismutase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Schacht, J Univ Michigan, Kresge Hearing Res Inst, Dept Otolaryngol, 1301 E Ann St, Ann Arbor, MI 48109 USA Univ Michigan 1301 E Ann St Ann Arbor MI USA 48109 MI 48109 USA
Citazione:
S.H. Sha et al., "Overexpression of copper/zinc-superoxide dismutase protects from kanamycin-induced hearing loss", AUDIOL NEUR, 6(3), 2001, pp. 117-123

Abstract

The participation of reactive oxygen species in aminoglycoside-induced ototoxicity has been deduced from observations that aminoglycoside-iron complexes catalyze the formation of superoxide radicals in vitro and that antioxidants attenuate ototoxicity in vivo. We therefore hypothesized that overexpression of Cu/Zn-superoxide dismutase (h-SOD1) should protect transgenic mice from ototoxicity. Immunocytochemistry confirmed expression of h-SOD1 in inner ear tissues of transgenic C57BL/6-TgN[SOD1]3Cje mice. Transgenic and nontransgenic littermates received kanamycin (400 mg/kg body weight/day) for 10 days beginning on day 10 after birth. Auditory thresholds were tested by evoked auditory brain stem responses at 1 month after birth. In nontransgenic animals, the threshold in the kanamycin-treated group was 45-50 dB higher than in saline-injected controls. In the transgenic group, kanamycin increased the threshold by only 15 dB over the respective controls. The effects were similar at 12 and 24 kHz. The protection by overexpression of superoxide dismutase supports the hypothesis that oxidant stress plays a significant role in aminoglycoside-induced ototoxicity. The results also suggest transgenic animals as suitable models to investigate the underlying mechanisms and possible strategies for prevention. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:49:25