Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Culture of porcine hepatocytes: The dogma of exogenous matrix revisited
Autore:
Lorenti, A; Barbich, M; Hidalgo, A; Hyon, SH; Sorroche, P; Guinle, A; Schenone, T; Chamoles, N; Argibay, P;
Indirizzi:
Hosp Italiano Buenos Aires, Inst Ciencias Basicas & Med Expt, RA-4240 Buenos Aires, DF, Argentina Hosp Italiano Buenos Aires Buenos Aires DF Argentina RA-4240 F, Argentina Fdn Estudio Enfermedades Neurometab, Buenos Aires, DF, Argentina Fdn Estudio Enfermedades Neurometab Buenos Aires DF Argentina Argentina
Titolo Testata:
ARTIFICIAL ORGANS
fascicolo: 7, volume: 25, anno: 2001,
pagine: 546 - 550
SICI:
0160-564X(200107)25:7<546:COPHTD>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT-RAT HEPATOCYTES; COLLAGEN GEL; TRANSPLANTATION; SPHEROIDS; GROWTH; CELLS; SUBSTRATA;
Keywords:
pig hepatocyte culture; lidocaine metabolism; diazepam metabolism; exogenous matrix;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Argibay, P Hosp Italiano Buenos Aires, Inst Ciencias Basicas & Med Expt, C1199ACL, RA-4240 Buenos Aires, DF, Argentina Hosp Italiano Buenos Aires C1199ACL Buenos Aires DF Argentina RA-4240
Citazione:
A. Lorenti et al., "Culture of porcine hepatocytes: The dogma of exogenous matrix revisited", ARTIF ORGAN, 25(7), 2001, pp. 546-550

Abstract

The use of exogenous matrices has been described as an essential componentin securing the viability and functionality of hepatocytes in vitro whether cultured for extracorporeal devices or cell transplantation. Here we report on the in vitro culture of porcine hepatocytes in polystyrene tissue-culture flasks without exogenous matrices showing adequate attachment and viability. Cell proliferation was evidenced by uptake of 5-bromo-2'-deoxyuridine, with peaks at Days 2 (19.7 +/- 8.5%), 15 (20.8 +/- 3.3%), and 35 (21.4 +/- 0.3%). Detoxification capacity was assessed by determination of monoethylglycinexylidide, a product of lidocaine metabolism (highest value 156.5 +/- 10.1 ng/ml at Day 4), and by diazepam clearance (maximum mum clearance 66.2% at Day 6). Diazepam metabolite levels were highest at Day 4 both for temazepam and oxazepam (6.5 +/- 0.1 and 0.10 +/- 0.01, respectively). These results suggest that the need for an exogenous matrix to achieve sustained proliferative activity and differentiated hepatocyte function should not necessarily be considered a sine qua non condition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 04:49:34