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Titolo:
Effect of the neuroprotective agent riluzole on intracellular Ca2+ levels in IMR32 neuroblastoma cells
Autore:
Wang, JL; Lee, KC; Tang, KY; Lu, T; Chang, CH; Chow, CK; Chen, WC; Su, W; Law, YP; Jan, CR;
Indirizzi:
Kaohsiung Vet Gen Hosp, Dept Med Educ & Res, Kaohsiung, Taiwan Kaohsiung Vet Gen Hosp Kaohsiung Taiwan d Educ & Res, Kaohsiung, Taiwan Kaohsiung Vet Gen Hosp, Dept Internal Med, Kaohsiung, Taiwan Kaohsiung VetGen Hosp Kaohsiung Taiwan Internal Med, Kaohsiung, Taiwan Kaohsiung Vet Gen Hosp, Dept Psychiat, Kaohsiung, Taiwan Kaohsiung Vet GenHosp Kaohsiung Taiwan ept Psychiat, Kaohsiung, Taiwan Ping Tung Christian Hosp, Div Urol, Ping Tung, Taiwan Ping Tung Christian Hosp Ping Tung Taiwan , Div Urol, Ping Tung, Taiwan Pao Chien Gen Hosp, Dept Pediat, Ping Tung, Taiwan Pao Chien Gen Hosp Ping Tung Taiwan osp, Dept Pediat, Ping Tung, Taiwan Pao Chien Gen Hosp, Dept Med, Ping Tung, Taiwan Pao Chien Gen Hosp Ping Tung Taiwan n Hosp, Dept Med, Ping Tung, Taiwan Natl Sun Yat Sen Univ, Inst Life Sci, Kaohsiung, Taiwan Natl Sun Yat Sen Univ Kaohsiung Taiwan Inst Life Sci, Kaohsiung, Taiwan Natl Sun Yat Sen Univ, Dept Biol, Kaohsiung, Taiwan Natl Sun Yat Sen UnivKaohsiung Taiwan iv, Dept Biol, Kaohsiung, Taiwan Natl Yang Ming Univ, Sch Med, Taipei, Taiwan Natl Yang Ming Univ Taipei Taiwan ng Ming Univ, Sch Med, Taipei, Taiwan Kaohsiung Vet Gen Hosp, Dept Rehabil, Kaohsiung, Taiwan Kaohsiung Vet Gen Hosp Kaohsiung Taiwan Dept Rehabil, Kaohsiung, Taiwan
Titolo Testata:
ARCHIVES OF TOXICOLOGY
fascicolo: 4, volume: 75, anno: 2001,
pagine: 214 - 220
SICI:
0340-5761(200106)75:4<214:EOTNAR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; ACTIVATED CALCIUM CHANNELS; ADRENAL CHROMAFFIN CELLS; ROOT GANGLION NEURONS; SPINAL-CORD INJURY; METHYL-D-ASPARTATE; DOPAMINE RELEASE; CORTICAL-NEURONS; SODIUM-CHANNELS; RAT;
Keywords:
Ca2+; Ca2+ stores; fura-2; IMR32; neuroblastoma cells; riluzole;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Jan, CR Kaohsiung Vet Gen Hosp, Dept Med Educ & Res, 386 Ta Chung 1st Rd, Kaohsiung, Taiwan Kaohsiung Vet Gen Hosp 386 Ta Chung 1st Rd Kaohsiung Taiwan iwan
Citazione:
J.L. Wang et al., "Effect of the neuroprotective agent riluzole on intracellular Ca2+ levels in IMR32 neuroblastoma cells", ARCH TOXIC, 75(4), 2001, pp. 214-220

Abstract

Riluzole is an effective neuroprotective drug. Its effect on intracellularfree Ca2+ levels ([Ca2+](i)) has not been explored. This study examined the effect of riluzole on [Ca2+](i) in IMR32 neuroblastoma cells using fura-2as a Ca2+ probe. Riluzole 0.1-1 mM increased [Ca2+](i) in a concentration-dependent manner. Removal of extracellular Ca2+ inhibited the response by 52 +/- 5%. The [Ca2+](i) increase induced by 0.2 mM riluzole was unaltered by 0.1 mM La3+ or 10 muM verapamil, but was inhibited by 51 +/- 4% by 10 muMnifedipine. In Ca2+ -free medium, pretreatment with 1 muM thapsigargin (anendoplasmic reticulum Ca2+ pump inhibitor) reduced the 0.2 mM riluzole-induced Ca2+ release by 44 +/-3%; this reduction was augmented to 66 +/-5% by additionally depleting the Ca2+ stores in the Golgi complex with 50 muM brefeldin A. Inhibition of inositol 1,4,5-trisphosphate formation by 2 muM U73122, a phospholipase C inhibitor. did not affect Ca2+ release induced by 0.2 muM riluzole. It was concluded that the neuroprotective agent riluzole increased [Ca2+](i) in IMR32 neuroblastoma cells concentration-dependently byreleasing Ca2+ from multiple stores in an inositol 1,4,5-trisphosphate-independent manner and also by inducing nifedipine-sensitive Ca2+ influx.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:35:07