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Titolo:
Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter
Autore:
Maruyama, Y; Suzuki, Y; Kazusaka, A; Fujita, S;
Indirizzi:
Hokkaido Univ, Grad Sch Vet Med, Dept Environm Vet Sci, Toxicol Lab, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan
Titolo Testata:
ARCHIVES OF TOXICOLOGY
fascicolo: 4, volume: 75, anno: 2001,
pagine: 209 - 213
SICI:
0340-5761(200106)75:4<209:UOTDNN>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
N-METHYL-NORSALSOLINOL; HUNTINGTONS-DISEASES; MICE LACKING; SALSOLINOL; PARKINSONISM; DERIVATIVES; MPTP; 6-HYDROXYDOPAMINE; SAMPLES; SYSTEM;
Keywords:
dopamine transporter; norsalsolinol; parkinsonism; PC12 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Fujita, S Hokkaido Univ, Grad Sch Vet Med, Dept Environm Vet Sci, Toxicol Lab, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan0600818 ido 0600818, Japan
Citazione:
Y. Maruyama et al., "Uptake of the dopaminergic neurotoxin, norsalsolinol, into PC12 cells via dopamine transporter", ARCH TOXIC, 75(4), 2001, pp. 209-213

Abstract

The uptake of norsalsolinol, a neurotoxin candidate causing parkinsonism-like symptoms, was studied in PC 12 cells. The compound was actively taken up by the PC 12 cells, with a K-m value of 176.2 +/- 9.1 muM and a maximum velocity of 55.6 +/- 7.0 pmol/min per mg protein; norsalsolinol uptake was dependent on the presence of extracellular Na+. The uptake of norsalsolinol was sensitive to two dopamine transporter inhibitors, GBR-12909 and reserpine, but was less sensitive to desipramine, a noradrenaline transporter inhibitor. Dopamine competitively inhibited norsalsolinol uptake into PC12 cells with a K-i value of 271.2 +/- 61.6 muM. These results suggest that norsalsolinol is taken up into PC12 cells mainly by the dopamine transporter.

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Documento generato il 17/09/19 alle ore 22:36:59