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Titolo:
Improvement and validation of the fluorescence-based histone deacetylase assay using an internal standard
Autore:
Hoffmann, K; Heltweg, B; Jung, M;
Indirizzi:
Univ Munster, Dept Pharmaceut Chem, D-48149 Munster, Germany Univ MunsterMunster Germany D-48149 ceut Chem, D-48149 Munster, Germany
Titolo Testata:
ARCHIV DER PHARMAZIE
fascicolo: 7, volume: 334, anno: 2001,
pagine: 248 - 252
SICI:
0365-6233(200107)334:7<248:IAVOTF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL CONTROL; PROMYELOCYTIC LEUKEMIA; TRICHOSTATIN-A; CANCER-CELLS; INHIBITORS; CHROMATIN; REGULATOR; TRAPOXIN; POTENT;
Keywords:
histone deaceylase; fluorogenic substrate; coumarin; trichostatin A; HC toxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Jung, M Univ Munster, Dept Pharmaceut Chem, Hittorfstr 58-62, D-48149 Munster, Germany Univ Munster Hittorfstr 58-62 Munster Germany D-48149 er, Germany
Citazione:
K. Hoffmann et al., "Improvement and validation of the fluorescence-based histone deacetylase assay using an internal standard", ARCH PHARM, 334(7), 2001, pp. 248-252

Abstract

The determination of the activity of histone deacetylase (HDAC) and the potency of its inhibitors has become an important goal in medicinal chemistry. This is due both to the involvement of HDAC in gene regulation and the ability of its inhibitors to modulate transcription and induce differentationand/or apoptosis in cancer cells. We have previously reported the development of a non-isotopic assay for HDAC using a fluorescent derivative of e-acetyl lysine. It can replace existing methods that rely on radioactively labeled histories or oligopeptides as substrates. Here we report validation and improvement of the procedure using an internal standard for the quantitation of the fluorescent substrate by HPLC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 00:39:42