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Titolo:
Subtyping of Campylobacter spp. strains and their incidence in piglets
Autore:
Steinhauserova, I; Fojtikova, K; Matiasovic, J;
Indirizzi:
Univ Vet & Pharmaceut Sci, Dept Meat Hyg & Technol, Fac Vet Hyg & Ecol, Brno 61242, Czech Republic Univ Vet & Pharmaceut Sci Brno Czech Republic 61242 1242, Czech Republic Univ Vet & Pharmaceut Sci, Dept Anim Breeding & Genet, Fac Vet Hyg & Ecol,Brno, Czech Republic Univ Vet & Pharmaceut Sci Brno Czech Republic Ecol,Brno, Czech Republic
Titolo Testata:
ACTA VETERINARIA BRNO
fascicolo: 2, volume: 70, anno: 2001,
pagine: 197 -
SICI:
0001-7213(200106)70:2<197:SOCSSA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREVALENCE; JEJUNI; PIGS;
Keywords:
Campylobacter coli; Campylobacter jejuni; typing; PCR-RFLP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Steinhauserova, I Univ Vet & Pharmaceut Sci, Dept Meat Hyg & Technol, Fac Vet Hyg & Ecol, Palackeho 1-3, Brno 61242, Czech Republic Univ Vet & Pharmaceut Sci Palackeho 1-3 Brno Czech Republic 61242
Citazione:
I. Steinhauserova et al., "Subtyping of Campylobacter spp. strains and their incidence in piglets", ACT VET B, 70(2), 2001, pp. 197

Abstract

Campylobacter spp. are common cause of enteritis in humans, but the routesof transmission and the subtypes of these bacteria responsible for infection are not clearly known, Pigs, which are infected early in life, are knownto be symptomless carriers of Campylobacter spp. We evaluated and subtyped225 samples of rectal swabs from piglets with and without disease by polymerase chain reaction (PCR) and restriction fragment length polymorphism. Little difference was found in the presence of Campylobacter spp. in healthy and diseased piglets: Campylobacter sp. was found in 49% of 225 samples of rectal swabs from piglets with enteric disease, C. coli, C. jejuni and atypical strains representing 81%, 17% and 2%, respectively, and in 41% of rectal swabs from healthy, piglets, C. coli and C. jejuni representing 85% and 15%, respectively. C. coli strains were subtyped from the PCR product with the Hinf1 restriction enzyme, and four subtypes were found and confirmed bysequencing. The most frequent were types III (46%) and I (31%), whereas type II was found in 6% of strains only, and 17% of strains had no cutting site (type IV). Thus, both healthy and sick piglets had a high incidence of Campylobacter spp., which might result in contamination of pork during slaughter. No link was demonstrated between the incidence of individual subtypesof C. coli and the frequency or severity of disease on pig farms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 11:15:47