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Titolo:
Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depresses
Autore:
Unuma, T; Okamoto, H; Konishi, K; Ohta, H; Mori, K;
Indirizzi:
Natl Res Inst Aquaculture, Nansei, Mie 5160193, Japan Natl Res Inst Aquaculture Nansei Mie Japan 5160193 ei, Mie 5160193, Japan Natl Res Inst Aquaculture, Inland Stn, Tamaki, Mie 5190423, Japan Natl ResInst Aquaculture Tamaki Mie Japan 5190423 ki, Mie 5190423, Japan Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan Tohoku Univ Sendai Miyagi Japan 9818555 ci, Sendai, Miyagi 9818555, Japan
Titolo Testata:
ZOOLOGICAL SCIENCE
fascicolo: 4, volume: 18, anno: 2001,
pagine: 559 - 565
SICI:
0289-0003(200105)18:4<559:COCEVA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATING CELL-ADHESION; MAJOR YOLK GLYCOPROTEIN; PRECURSOR; CELOMOCYTES; ECHINOIDS; COMPLEX; PROTEIN; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Unuma, T Natl Res Inst Aquaculture, Nansei, Mie 5160193, Japan Natl Res Inst Aquaculture Nansei Mie Japan 5160193 160193, Japan
Citazione:
T. Unuma et al., "Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depresses", ZOOL SCI, 18(4), 2001, pp. 559-565

Abstract

Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of gametogenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-length cDNA encoding vitellogenin (Vg; the MYP precursor in the coelomic fluid), and investigated its expression in various tissues of immature adults. The nucleotide sequence of Vg contains an open reading frame of 4050 by encoding 1349 amino acids. The deduced amino acid sequence near the N-terminal showed 25% homology to the vertebrate transferrin family. Vitellogenin mRNA was detected in the ovary, testis, stomach, intestine and rectum by Northern blot analysis, with the highest level of mRNA expression in the gonad. Weak expression was also detected in the esophagus and coelomocytes by RT-PCR. In situ hybridization demonstrated that nutritive phagocytes, which exclusively fill the lumina of the immature gonad, contained Vg mRNA. These results suggested that the MYP stored in the immature gonads is synthesized andaccumulated mainly within the nutritive phagocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 17:48:29