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Titolo:
12-O-tetradecanoylphorbol-13-acetate induces Epstein-Barr virus reactivation via NF-kappa B and AP-1 as regulated by protein kinase C and mitogen-activated protein kinase
Autore:
Gao, X; Ikuta, K; Tajima, M; Sairenji, T;
Indirizzi:
Tottori Univ, Fac Med, Sch Life Sci, Dept Biosignaling, Yonago, Tottori 6838503, Japan Tottori Univ Yonago Tottori Japan 6838503 Yonago, Tottori 6838503, Japan Teikyo Univ, Sch Med, Cent Clin Lab, Tokyo 1738606, Japan Teikyo Univ Tokyo Japan 1738606 Med, Cent Clin Lab, Tokyo 1738606, Japan
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 286, anno: 2001,
pagine: 91 - 99
SICI:
0042-6822(20010720)286:1<91:1IEVR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; EPITHELIAL-CELL LINES; ESCHERICHIA-COLI LIPOPOLYSACCHARIDE; LYMPHOCYTE GROWTH TRANSFORMATION; CULTURED MURINE HEPATOCYTES; LATENT MEMBRANE-PROTEIN-1; DIMERIZATION DOMAIN; ZTA TRANSACTIVATOR; GENE-EXPRESSION; LEUCINE ZIPPER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Sairenji, T Tottori Univ, Fac Med, Sch Life Sci, Dept Biosignaling, Yonago, Tottori 6838503, Japan Tottori Univ Yonago Tottori Japan 6838503 tori 6838503, Japan
Citazione:
X. Gao et al., "12-O-tetradecanoylphorbol-13-acetate induces Epstein-Barr virus reactivation via NF-kappa B and AP-1 as regulated by protein kinase C and mitogen-activated protein kinase", VIROLOGY, 286(1), 2001, pp. 91-99

Abstract

Signaling pathway components mediating Epstein-Barr virus (EBV) reactivation by 12-O-tetradecanoylphorbol-13-acetate (TPA) were characterized in terms of induction and modification of specific transacting factors. The consequences of protein kinase C (PKC) activation by TPA in inhibiting inducible nitric oxide synthase (iNOS) mRNA expression were analyzed in the EBV-infected gastric epithelial cell line GT38. Spontaneous expression of the EBV BZLF1 gene product ZEBRA became undetectable upon long-term culturing of GT38cells, while iNOS mRNA expression increased. In such cells the PKC inhibitors 1-(5-isoquinolinesulphonyl)-2,5-dimethylpiperazine (H7) and staurosporine inhibited TPA-induced expression of BZLF1 and BRLF1 and reversed TPA-mediated inhibition of iNOS gene expression. The mitogen-activated protein kinase inhibitor PD98059 inhibited TPA-induced BZLF1 expression. Electrophoretic mobility shift assays demonstrated that transcription factors NF-kappaB and AP-1 were also activated by TPA in a time-dependent manner. The TPA-induced NF-kappaB activation was inhibited by prior treatment of the cells with the NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC). TPA-induced BZLF1 expression was also inhibited by the treatment with PDTC. Northern blot analyses characterized changes in levels of the c-jun and junB expressions of the AP-1 family. These results show that TPA induces EBV reactivation via NF-kappaB and AP-1 and that PKC is an important mediator in regulating gene expression leading to EBV reactivation after TPA treatment of GT38 cells. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 09:52:02