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Titolo:
Nuclear localization of the Saccharomyces cerevisiae HMG protein NHP6A occurs by a Ran-independent nonclassical pathway
Autore:
Yen, YM; Roberts, PM; Johnson, RC;
Indirizzi:
Univ Calif Los Angeles, Dept Biol Chem, Sch Med, Los Angeles, CA 90095 USAUniv Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Rochester, Dept Biol, Rochester, NY 14627 USA Univ Rochester Rochester NY USA 14627 Dept Biol, Rochester, NY 14627 USA Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
TRAFFIC
fascicolo: 7, volume: 2, anno: 2001,
pagine: 449 - 464
SICI:
1398-9219(200107)2:7<449:NLOTSC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOBILITY GROUP PROTEINS; GREEN FLUORESCENT PROTEIN; NUCLEOCYTOPLASMIC TRANSPORT; DNA-BINDING; PORE COMPLEX; IN-VIVO; ENVELOPE PERMEABILITY; BETA-CATENIN; LARGE-T; YEAST;
Keywords:
diffusion channel; green fluorescent protein; importins/karyopherins; nuclear import signals; nuclear pore complex; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
85
Recensione:
Indirizzi per estratti:
Indirizzo: Johnson, RC Univ Calif Los Angeles, Dept Biol Chem, Sch Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 CA 90095 USA
Citazione:
Y.M. Yen et al., "Nuclear localization of the Saccharomyces cerevisiae HMG protein NHP6A occurs by a Ran-independent nonclassical pathway", TRAFFIC, 2(7), 2001, pp. 449-464

Abstract

The Saccharomyces cerevisiae non-histone protein 6-A (NHP6A) is a member of the high-mobility group 1/2 protein family that bind and bend DNA of mixed sequence. NHP6A has only one high-mobility group 1/2 DNA binding domain and also requires a 16-amino-acid basic tail at its N-terminus for DNA binding. We show in this report that nuclear accumulation of NHP6A is strictly correlated with its DNA binding properties since only nonhistone protein 6 A-green fluorescent protein chimeras that were competent for DNA binding were localized to the nucleus. Despite the requirement for basic residues within the N-terminal segment for DNA binding and nuclear accumulation, this region does not appear to contain a nuclear localization signal. Moreover, NHP6A does not bind to the yeast nuclear localization signal receptor SRP1 and nuclear targeting of NHP6A does not require the function of the 14 different importins. Unlike histone H2B1 which contains a classical nuclear localization signal, entry of NHP6A into the nucleus was found to be independentof Ran as judged by coexpression of Ran GTPase mutants and was shown to occur at 0 degreesC after a 15-min induction. These unusual properties lead us to suggest that NHP6A entry into the nucleus proceeds by a nonclassical Ran-independent pathway.

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Documento generato il 03/04/20 alle ore 11:11:12