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Titolo:
C-13-NMR of forest soil lipids
Autore:
Almendros, G; Tinoco, P; Gonzalez-Vila, FJ; Ludemann, HD; Sanz, J; Velasco, F;
Indirizzi:
CSIC, Ctr Ciencias Medioambinetales, E-28006 Madrid, Spain CSIC Madrid Spain E-28006 encias Medioambinetales, E-28006 Madrid, Spain CSIC, Inst Recursos Nat & Agrobiol, E-41080 Seville, Spain CSIC Seville Spain E-41080 cursos Nat & Agrobiol, E-41080 Seville, Spain Inst Biophys & Phys Biochem, D-93040 Regensburg, Germany Inst Biophys & Phys Biochem Regensburg Germany D-93040 gensburg, Germany CSIC, Inst Quim Organ Gen, E-28006 Madrid, Spain CSIC Madrid Spain E-28006 IC, Inst Quim Organ Gen, E-28006 Madrid, Spain
Titolo Testata:
SOIL SCIENCE
fascicolo: 3, volume: 166, anno: 2001,
pagine: 186 - 196
SICI:
0038-075X(200103)166:3<186:COFSL>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYPRENOLS; KEROGEN; PLANTS; NMR;
Keywords:
lipids; alkanes; fatty acids; resin acids; polyprenols; pine; oak; juniper;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Almendros, G CSIC, Ctr Ciencias Medioambinetales, Serrano 115 B, E-28006 Madrid, Spain CSIC Serrano 115 B Madrid Spain E-28006 -28006 Madrid, Spain
Citazione:
G. Almendros et al., "C-13-NMR of forest soil lipids", SOIL SCI, 166(3), 2001, pp. 186-196

Abstract

Molecular characterization of soil lipids often provides valuable biogeochemical information about the impact of vegetation, microorganisms, and abiotic factors on the soil C sequestration proccess. The total lipid extractedwith petroleum ether from nine soils developed under three types of Mediterranean forest (stone pine (Pinus pinea L.), evergreen oak (Quercus rotundifolia L.), and Spanish juniper (Juniperus thurifera L)) has been analyzed by high-resolution C-13 nuclear magnetic resonance (C-13-NMR) under quantitative acquisition conditions. Tentative assignments of the spectral peaks are presented, and the spectra of soil lipids are compared with those from the lipids extracted directly from leaves of the corresponding trees. This comparison evidenced that soil lipids behaved as biomarker soil fractions when analyzed by 13C-NMR as a whole. Analysis by gas chromatography-mass spectrometry (GC/MS) reveals that the volatile fraction of the lipid extract (46%, on average, as estimated by internal reference) consisted mainly of freealkanes, alkanoic acids (<C-30), and diterpene resin acids. We observed some differences between the chemical structures suggested by 13C-NMR and GC/MS. This was interpreted as a portion of soil and plant lipids consisting of extractable material that cannot be detected by standard GC methods. The complex signal pattern in the 0 to 30 ppm chemical shift range showed typical signals for carbons in acyl polymethylene chains, which overlapped with a pattern suggesting isoprenoid-like branching in long-chainstructures (major signals at ca. 22, 26, and 32 ppm). In addition, periodic unsaturations suggested by signals at ca. 124 and 135 ppm are also compatible with polyprenoid-type backbones. The alkyl region coincided with thoseof mono- to triacyl glycerol fatty esters. It seems evident that 13C-NMR allows us to characterize structures present in nonvolatile complex material.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 05:44:21