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Titolo:
Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein
Autore:
Abe, M; Takahashi, T; Komeda, Y;
Indirizzi:
Hokkaido Univ, Div Biol Sci, Grad Sch Sci, Sapporo, Hokkaido 0600810, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600810 oro, Hokkaido 0600810, Japan
Titolo Testata:
PLANT JOURNAL
fascicolo: 5, volume: 26, anno: 2001,
pagine: 487 - 494
SICI:
0960-7412(200106)26:5<487:IOACEF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHOOT APICAL MERISTEM; ARABIDOPSIS-THALIANA; HOMEOBOX GENE; CELL-DIFFERENTIATION; PLANT DEVELOPMENT; VEGETATIVE SHOOT; ROOT; MAINTENANCE; EPIDERMIS; PATTERNS;
Keywords:
Arabidopsis thaliana; L1 layer; PROTODERMAL FACTOR 1; cis-regulatory element; L1 box;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, T Hokkaido Univ, Div Biol Sci, Grad Sch Sci, N10,W8, Sapporo, Hokkaido 0600810, Japan Hokkaido Univ N10,W8 Sapporo Hokkaido Japan 0600810 10, Japan
Citazione:
M. Abe et al., "Identification of a cis-regulatory element for L1 layer-specific gene expression, which is targeted by an L1-specific homeodomain protein", PLANT J, 26(5), 2001, pp. 487-494

Abstract

The Arabidopsis thaliana PROTODERMAL FACTOR1 (PDF1) gene encoding a putative extracellular proline-rich protein is exclusively expressed in the L1 layer of shoot apices and the protoderm of organ primordia. In order to identify essential cis-regulatory sequences required for the L1 layer-specific expression, a series of 5' deletions of the PDF1 promoter were fused to the beta -glucronidase (GUS) gene and introduced into Arabidopsis plants. Our analysis revealed that the minimum region necessary to confer L1-specific expression of PDF1 is confined within a 260-bp fragment upstream of the transcription start site. We identified an 8-bp motif in this region that is conserved between promoter regions of all the L1-specific genes so far cloned,and we designated it the L1 box. Electrophoretic mobility shift assays demonstrated that the L1-specific homeodomain protein ATML1 can bind to the L1box sequence in vitro. The GUS expression in transgenic plants disappearedwhen a mutation that abolishes binding of ATML1 was introduced into the PDF1 /1 box sequence of the construct. These results suggest that the L1 box plays a crucial role in the regulation of PDF1 expression in L1 cells and that ATML1 could cooperate to drive L1-specific expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:50:17