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Titolo:
Physical interactions between ABA response loci of Arabidopsis
Autore:
Nakamura, S; Lynch, TJ; Finkelstein, RR;
Indirizzi:
Univ Calif Santa Barbara, Mol Cellular & Dev Biol Dept, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 Barbara, CA 93106 USA
Titolo Testata:
PLANT JOURNAL
fascicolo: 6, volume: 26, anno: 2001,
pagine: 627 - 635
SICI:
0960-7412(200106)26:6<627:PIBARL>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-INDUCED TRANSCRIPTION; CONSERVED B3 DOMAIN; ABSCISIC-ACID; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; VIVIPAROUS1 VP1; EM PROMOTER; BZIP FACTOR; ABI3; PROTEIN;
Keywords:
ABA-insensitive (ABI) loci; ABA response; Arabidopsis; two-hybrid assays; bZIP factors; B3 domain factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Finkelstein, RR Univ Calif Santa Barbara, Mol Cellular & Dev Biol Dept, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara Santa Barbara CA USA 93106 6 USA
Citazione:
S. Nakamura et al., "Physical interactions between ABA response loci of Arabidopsis", PLANT J, 26(6), 2001, pp. 627-635

Abstract

Genetic and physiological studies have shown that the Arabidopsis thalianaabscisic acid-insensitive (ABi) loci interact to regulate seed-specific and/or ABA-inducible gene expression. We have used the yeast two-hybrid assayto determine whether any of these genetic interactions reflect direct physical interactions. By this criterion, only ABI3 and ABI5 physically interact with each other, and ABI5 can form homodimers. The B1 domain of ABI3 is essential for this interaction; this is the first specific function ascribedto this domain of the ABI3/VP1 family. The ABI5 domains required for interaction with ABI3 include two conserved charged domains in the amino-terminal half of the protein. An additional conserved charged domain appears to have intrinsic transcription activation function in this assay. Yeast one-hybrid assays with a lacZ reporter gene under control of the late embryogenesis-abundant AtEm6 promoter show that only ABI5 binds directly to this promoter fragment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:17:44