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Titolo:
Rerouting the plant phenylpropanoid pathway by expression of a novel bacterial enoyl-CoA hydratase/lyase enzyme function
Autore:
Mayer, MJ; Narbad, A; Parr, AJ; Parker, ML; Walton, NJ; Mellon, FA; Michael, AJ;
Indirizzi:
AFRC, Inst Food Res, Div Food Safety Sci, Norwich NR4 7UA, Norfolk, England AFRC Norwich Norfolk England NR4 7UA i, Norwich NR4 7UA, Norfolk, England
Titolo Testata:
PLANT CELL
fascicolo: 7, volume: 13, anno: 2001,
pagine: 1669 - 1682
SICI:
1040-4651(200107)13:7<1669:RTPPPB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
CINNAMYL ALCOHOL-DEHYDROGENASE; TRANSGENIC TOBACCO PLANTS; ACID O-METHYLTRANSFERASE; HAIRY ROOT CULTURES; DOWN-REGULATION; DISEASE RESISTANCE; GENE-EXPRESSION; INITIATOR CODON; MALE-STERILITY; FERULIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Michael, AJ AFRC, Inst Food Res, Div Food Safety Sci, Norwich Res Pk, Norwich NR4 7UA,Norfolk, England AFRC Norwich Res Pk Norwich Norfolk England NR4 7UA k, England
Citazione:
M.J. Mayer et al., "Rerouting the plant phenylpropanoid pathway by expression of a novel bacterial enoyl-CoA hydratase/lyase enzyme function", PL CELL, 13(7), 2001, pp. 1669-1682

Abstract

The gene for a bacterial enoyl-CoA hydratase (crotonase) homolog (HCHL) previously shown to convert 4-coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA to the corresponding hydroxybenzaldehydes in vitro provided an opportunity to subvert the plant phenylpropanoid pathway and channel carbon flux through4-hydroxybenzaldehyde and the important flavor compound 4-hydroxy-3-methoxybenzaldehyde (vanillin), Expression of the Pseudomonas fluorescens AN103 HCHL gene in two generations of tobacco plants caused the development of phenotypic abnormalities, including stunting, interveinal chlorosis and senescence, curled leaf margins, low pollen production, and male sterility. In second generation progeny, the phenotype segregated with the transgene and transgenic siblings exhibited orange/red coloration of the vascular ring, distorted cells in the xylem and phloem bundles, and lignin modification/reduction. There was depletion of the principal phenolics concomitant with massive accumulation of novel metabolites, including the glucosides and glucose esters of 4-hydroxybenzoic acid and vanillic acid and the glucosides of 4-hydroxybenzyl alcohol and vanillyl alcohol, HCHL plants exhibited increased accumulation of transcripts for phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, and 4-coumarate:CoA ligase, whereas beta -1,3-glucanase was suppressed, This study, exploiting the ability of a bacterial gene to divert plant secondary metabolism, provides insight into how plants modify inappropriately accumulated metabolites and reveals the consequences of depleting the major phenolic pools.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:51:16