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Titolo:
Protection from paraquat-mediated photo-oxidative stress by glutathione inpoplar (Populus tremula x P-alba) plants
Autore:
Will, B; Jouanin, L; Rennenberg, H;
Indirizzi:
Univ Freiburg, Inst Forstbot & Baumphysiol, D-79110 Freiburg, Germany UnivFreiburg Freiburg Germany D-79110 hysiol, D-79110 Freiburg, Germany Inst Natl Rech Agron, Lab Biol Cellulaire, Versailles, France Inst Natl Rech Agron Versailles France l Cellulaire, Versailles, France
Titolo Testata:
PLANT BIOLOGY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 272 - 278
SICI:
1435-8603(200105)3:3<272:PFPPSB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; PHOTOOXIDATIVE STRESS; TRANSGENIC PLANTS; ACTIVE OXYGEN; TOLERANCE; LEAVES; REDUCTASE; CHLOROPLASTS; RESISTANCE; ASCORBATE;
Keywords:
glutathione; oxidative stress; paraquat; poplar; stress protection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Rennenberg, H Univ Freiburg, Inst Forstbot & Baumphysiol, Georges Kohler Allee 053-054, D-79110 Freiburg, Germany Univ Freiburg Georges Kohler Allee 053-054 Freiburg Germany D-79110
Citazione:
B. Will et al., "Protection from paraquat-mediated photo-oxidative stress by glutathione inpoplar (Populus tremula x P-alba) plants", PLANT BIO, 3(3), 2001, pp. 272-278

Abstract

The sensitivity of hybrid poplar (Populus tremula x P. alba) to oxidative stress mediated by paraquat exposure was analysed with leaf discs from wild-type plants and plants expressing the bacterial cDNA of the enzymes of glutathione synthesis, namely gshI, encoding gamma -glutamylcysteine synthetase (ECS), or gshII, encoding glutathione synthetase (GS), both in the cytosol. It was expected that leaf discs containing more than 2-fold elevated glutathione concentrations due to over-expression of ECS are less susceptible to paraquat exposure than wildtype plants and transformants over-expressingGS. However, neither over-expression of GS nor of ECS improved paraquat tolerance of the leaves. This result was surprising, because in wildtype plants reduced paraquat sensitivity of young compared with mature leaves coincided with co. 30% higher glutathione contents of the young leaves. Apparently, developmental changes in paraquat sensitivity of poplar leaves are controlled by factors different from glutathione contents. Feeding experiments with glutathione and its metabolic precursor gamma -glutamylcysteine (EC) plus gly showed that glutathione can provide protection from paraquat-mediated photo-oxidative stress; but at least co. 5-fold elevated glutathione levels are required for this effect in poplar leaves. Currently, such high glutathione levels have not been achieved by the application of plant molecularbiology techniques. The significance of glutathione for the compensation of photo-oxidative stress is discussed.

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Documento generato il 28/03/20 alle ore 13:12:09