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Titolo:
A glycoprotein from Schistosoma mansoni eggs binds non-antigen-specific immunoglobulin E and releases interleukin-4 from human basophils
Autore:
Haisch, K; Schramm, G; Falcone, FH; Alexander, C; Schlaak, M; Haas, H;
Indirizzi:
Forschungsinst Borstel, Div Cellular Allergol, D-23845 Borstel, Germany Forschungsinst Borstel Borstel Germany D-23845 D-23845 Borstel, Germany SUNY Buffalo, Dept Microbiol, Buffalo, NY 14260 USA SUNY Buffalo Buffalo NY USA 14260 , Dept Microbiol, Buffalo, NY 14260 USA Univ Edinburgh, Inst Cell Anim & Populat Biol, Edinburgh, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland burgh, Midlothian, Scotland
Titolo Testata:
PARASITE IMMUNOLOGY
fascicolo: 8, volume: 23, anno: 2001,
pagine: 427 - 434
SICI:
0141-9838(200108)23:8<427:AGFSME>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
HISTAMINE-RELEASE; HUMAN RESISTANCE; BLOOD BASOPHILS; IGE; PURIFICATION; IDENTIFICATION; RESPONSES; CHROMATOGRAPHY; SIALOPROTEIN; GRANULOMA;
Keywords:
SmEA; basophils; Schistosoma mansoni; IL-4; eggs; IgE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Haas, H Forschungsinst Borstel, Div Cellular Allergol, Parkallee 22, D-23845 Borstel, Germany Forschungsinst Borstel Parkallee 22 Borstel Germany D-23845 rmany
Citazione:
K. Haisch et al., "A glycoprotein from Schistosoma mansoni eggs binds non-antigen-specific immunoglobulin E and releases interleukin-4 from human basophils", PARASITE IM, 23(8), 2001, pp. 427-434

Abstract

We have recently shown that soluble extracts from Schistosoma mansoni eggs(SmEA) triggered basophils from nonsensitized donors to rapidly release interleukin (IL)-4. Assuming that this mechanism might play a role in vivo inbiasing the immune response towards a Th2 phenotype, we determined basic properties of the IL-4-inducing activity contained in SmEA. Sensitivity to pepsin digestion indicated protein nature. Binding to and specific elution from Concanavalin A-sepharose suggested that this protein contains mannose residues, thus being a glycoprotein. The IL-4-inducing activity was stable for 30 min at room temperature towards shifting the pH between 3 and 10. When incubated at 100 degreesC, it was stable at pH 3, but less stable at neutral and alkaline pH. Electroelution from an SDS-PAGE gel indicated an apparent molecular weight of the IL-4-inducing activity between 31 and 66 kDa. Although binding to purified human immunoglobulin E (IgE) and activating basophils IgE-dependently, SmEA appears to activate basophils in a non-antigen-specific way, since the cells were purified from noninfected donors. Because the IL-4-inducing activity was found to be released from eggs, it could be an important factor in the environment of the eggs skewing the immune response towards the Th2 phenotype.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 15:57:26