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Titolo:
Detection of differentially expressed genes in human colon carcinoma cellstreated with a selective COX-2 inhibitor
Autore:
Zhang, ZH; DuBois, RN;
Indirizzi:
Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Dept Med, Nashville, TN 37232 USA Vanderbilt Univ, Med Ctr, Dept Cell Biol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 Cell Biol, Nashville, TN 37232 USA Dept Vet Affairs Med Ctr, Nashville, TN 37232 USA Dept Vet Affairs Med Ctr Nashville TN USA 37232 , Nashville, TN 37232 USA
Titolo Testata:
ONCOGENE
fascicolo: 33, volume: 20, anno: 2001,
pagine: 4450 - 4456
SICI:
0950-9232(20010727)20:33<4450:DODEGI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSTEROIDAL ANTIINFLAMMATORY DRUGS; SUPPRESSION SUBTRACTIVE HYBRIDIZATION; PROTEIN-TYROSINE-PHOSPHATASE; COLORECTAL-CANCER CELLS; CYCLOOXYGENASE-2 INHIBITOR; MOLECULAR-CLONING; MESSENGER-RNA; CDNA CLONING; TUMOR-GROWTH; MOUSE MODEL;
Keywords:
cyclo-oxygenase-2; NSAID; colon cancer; subtraction cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: DuBois, RN Vanderbilt Univ, Med Ctr, Dept Med GI, MCN C-2104, Nashville, TN 37232 USA Vanderbilt Univ MCN C-2104 Nashville TN USA 37232 TN 37232 USA
Citazione:
Z.H. Zhang e R.N. DuBois, "Detection of differentially expressed genes in human colon carcinoma cellstreated with a selective COX-2 inhibitor", ONCOGENE, 20(33), 2001, pp. 4450-4456

Abstract

Numerous reports suggest that use of nonsteroidal anti-inflammatory drugs (NSAIDs) decrease mortality from colorectal cancer. To better understand all of the mechanisms underlying this effect, the global pattern of gene expression in colon carcinoma cells following treatment with NS-398, a selective cyclooxygenase-2 inhibitor was evaluated. We utilized suppression subtractive hybridization combined with differential screening to identify genes whose expression was affected following treatment. Among the subtracted cDNAfragments confirmed as differentially expressed, there were two which are known to be involved in the regulation of cell adhesion (human FAT and proto-cadherin-7). We identified two other genes whose levels were decreased and these are known to be involved in the regulation of cell proliferation (cyclin K and p-100). We identified additional genes which are involved in different signaling pathways which regulate programmed cell death (Dynamin 2,Pdcd4 and LIP.1). These results provide evidence that some of the effects of NS-398 on carcinoma cells may be due to modulation of genes which regulate programmed cell death, cell proliferation and cell-cell communication. Additional studies are underway to determine the biological function of the novel genes that were identified.

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Documento generato il 03/07/20 alle ore 01:33:17