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Titolo:
Blocking AMPA receptor desensitization prolongs spontaneous EPSC decay times and depolarizes H1 horizontal cells in carp retinal slices
Autore:
Hirasawa, H; Shiells, R; Yamada, M;
Indirizzi:
Electrotech Lab, Supermol Div, Tsukuba, Ibaraki 3058568, Japan ElectrotechLab Tsukuba Ibaraki Japan 3058568 uba, Ibaraki 3058568, Japan Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan Univ TsukubaTsukuba Ibaraki Japan 3058572 sukuba, Ibaraki 3058572, Japan Univ Coll London, Dept Physiol, Biophys Unit, London WC1E 6BT, England Univ Coll London London England WC1E 6BT Unit, London WC1E 6BT, England
Titolo Testata:
NEUROSCIENCE RESEARCH
fascicolo: 3, volume: 40, anno: 2001,
pagine: 217 - 225
SICI:
0168-0102(200107)40:3<217:BARDPS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TIGER SALAMANDER RETINA; ROD BIPOLAR CELLS; SPECTRAL SENSITIVITIES; SYNAPTIC TRANSMISSION; GLUTAMATE RECEPTORS; GAP-JUNCTIONS; VOLTAGE-CLAMP; CURRENTS; LIGHT; RESPONSES;
Keywords:
AMPA receptor; cyclothiazide; desensitization; horizontal cell; retina; sEPSC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Yamada, M Tokyo Metropolitan Inst Technol, Hino, Tokyo 1910065, Japan Tokyo Metropolitan Inst Technol Hino Tokyo Japan 1910065 Japan
Citazione:
H. Hirasawa et al., "Blocking AMPA receptor desensitization prolongs spontaneous EPSC decay times and depolarizes H1 horizontal cells in carp retinal slices", NEUROSCI RE, 40(3), 2001, pp. 217-225

Abstract

Desensitization of HI horizontal cell (H1 HC) glutamate receptors was investigated in carp retinal slices using cyclothiazide (CTZ), an inhibitor of AMPA receptor desensitization. 100 muM CTZ depolarized H1 HCs and increasedthe amplitude of light responses. without any prominent changes in their kinetics. Spontaneous EPSCs (sEPSCs) in H1 HCs were observed in the presenceof 2.5 mM heptanol, an uncoupling agent of gap junctions. 20 muM GYKI52466(an AMPA receptor antagonist) blocked the sEPSCs. consistent with the sEPSCs being mediated by AMPA receptors. 100 muM cobalt suppressed the Frequency of sEPSCs without changing their mean peak amplitude, suggesting that calcium-dependent transmitter release from cones was not affected by heptanol. CTZ increased the total inward charge transferred per sEPSC by increasing the sEPSC decay time constant twofold, without any significant change in their frequency and mean peak amplitude. This suggests that the depolarizing effect of CTZ on I-Il HCs was due to blocking desensitization of AMPA receptors. increasing the inward current induced by glutamate released From conesynaptic terminals. The desensitization of glutamate receptors may function to extend the dynamic range of H1 HC light responses. (C) 2001 Elsevier Science Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:36:47