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Titolo:
Polymorphonuclear leucocyte rheology and cytosolic Ca2+ content after activation in chronic renal failure
Autore:
Caimi, G; Canino, B; Vaccaro, F; Montana, M; Carollo, C; Oddo, G; Lo Presti, R;
Indirizzi:
Univ Palermo, Ist Clin Med & Malattie Cardiovasc, I-90133 Palermo, Italy Univ Palermo Palermo Italy I-90133 ie Cardiovasc, I-90133 Palermo, Italy Univ Palermo, Serv Nefrol & Emodialisi, I-90133 Palermo, Italy Univ Palermo Palermo Italy I-90133 & Emodialisi, I-90133 Palermo, Italy
Titolo Testata:
NEPHROLOGY
fascicolo: 3, volume: 6, anno: 2001,
pagine: 113 - 117
SICI:
1320-5358(200106)6:3<113:PLRACC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROGEN-PEROXIDE PRODUCTION; MEMBRANE FLUIDITY; OXIDATIVE-METABOLISM; LEUKOCYTE RHEOLOGY; DIABETES-MELLITUS; DIALYSIS PATIENTS; FREE CALCIUM; NEUTROPHILS; PHAGOCYTOSIS; PARAMETERS;
Keywords:
chronic renal failure; cytosolic Ca2+ concentration; membrane fluidity; polymorphonuclear leucocyte; polymorphonuclear leucocyte activation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Caimi, G Via Leonardo da Vinci 52, I-90145 Palermo, Italy Via Leonardo daVinci 52 Palermo Italy I-90145 5 Palermo, Italy
Citazione:
G. Caimi et al., "Polymorphonuclear leucocyte rheology and cytosolic Ca2+ content after activation in chronic renal failure", NEPHROLOGY, 6(3), 2001, pp. 113-117

Abstract

We evaluated polymorphonuclear leucocyte (PMN) flow properties in patientswith clinically stable chronic renal failure (CRF) and in control subjectsat baseline and after activation with 4-phorbol 12-myristate 13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). Initial relative flow rate (IRFR) and clogging particles (CPs) were obtained using the St. George's Filtrometer, and PMN membrane fluidity was assessed by marking PMNs with 1-(4-(trimethylamino)phenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH). PMN cytosolic Ca2+ concentration was determined by marking PMNs with Fura 2-AM. At baseline, CRF patients showed a significant increase only in PMN cytosolic Ca2+ content. After activation with PMA and fMLP, a decrease in IRFR andan increase in CP were observed in both control subjects and CRF patients,although the variation in IRFR present in the group of CRF patients was greater than in the control group. After activation with PMA and fMLP, we found a decrease in PMN membrane fluidity only in CRF patients, but no variation in PMN cytosolic Ca2+ concentration in either group was observed. These results provide evidence for PMN dysfunction in chronic renal failure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:38:20