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Titolo:
Paraffin immunoreactivity of CD10, CDw75, and bcl-6 in follicle center cell lymphoma
Autore:
Dunphy, CH; Polski, JM; Evans, HL; Gardner, LJ;
Indirizzi:
St Louis Univ, Hlth Sci Ctr, Dept Pathol, Div Hematopathol, St Louis, MO 63104 USA St Louis Univ St Louis MO USA 63104 Hematopathol, St Louis, MO 63104 USA
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 5-6, volume: 41, anno: 2001,
pagine: 585 -
SICI:
1042-8194(200105)41:5-6<585:PIOCCA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMAS; B-CELL; PROTEIN EXPRESSION; CHROMOSOMAL TRANSLOCATION; MONOCLONAL-ANTIBODIES; HYPERPLASIA; SECTIONS; TISSUE;
Keywords:
immunohistochemistry; CD10; CDw75; bcl-6; follicle center cell lymphoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Dunphy, CH St Louis Univ, Sch Med, Dept Pathol, 1402 S Grand St, St Louis,MO 63104 USA St Louis Univ 1402 S Grand St St Louis MO USA 63104 O 63104 USA
Citazione:
C.H. Dunphy et al., "Paraffin immunoreactivity of CD10, CDw75, and bcl-6 in follicle center cell lymphoma", LEUK LYMPH, 41(5-6), 2001, pp. 585

Abstract

Follicle center cell lymphoma(FCCL) has the following immunophenotype(IP):sIg+, Pan B+, CD1O+/-, CD5-, CD23-/+, CD43-, CD11c-, CD25-. In addition, reactivities of a malignant lymphoma with CDw75(LN-1) and bcl-6 are considered indicators of FCCL. Bcl-6 expression is common in Grade 1 FCCL (100%) and rare in other indolent B-cell lymphomas(BCL). In contrast, bcl-2 expression is common in FCCL (80%) and in other BCL subtypes. Since no previous study has correlated paraffin immunoreactivity(PIR) of CD10, CDw75, and bcl-6 in FCCL (Grades 1-3), this is this study's purpose. Twenty-nine FCCL's were identified and reviewed (6, Grade 1; 10, Grade 2; 13, Grade 3) from the Division of Hematopathology, St. Louis University. The diagnoses were based on morphology and immunohistochemistry(IH)(21 cases)+/- the flow cytometric IP(14 cases). The paraffin blocks were stained forCD10 (Novacastra, Vector Laboratories, Burlingame, CA), CDw75 and bcl-6 (DAKO Corporation, Carpinteria, CA). Results showed that, CD10 by paraffin IH(PIH) was positive in 23 [18(strong); 3(moderate); 2(weak)] and negative in6(3, Grade 2; 3, Grade 3). All CD10-cases were CDw75+; 4, bcl-6+. The two CD10-, bcl-6-cases were Grade 2. CDw75 was positive in 28 cases [16(strong); Il(moderate); 1(weak)] and negative in 1 (Grade 3; CD10+, bcl-2+, bcl-6+). Bcl-6 was positive in 26 [16(strong); 6(moderate); 4(weak)] and negative in 3(Grade 2's). Thus, the sensitivity of CD10, CDw75, and bcl-6 by PM for FCCL was 79%, 97%, and 90%, respectively. Of the three stains evaluated by PIH in FCCL, CDw75 was the most sensitive, closely followed by bcl-6. CD10 was least sensitive-79%. By combining these 3 stains, the sensitivity was 100%; thus, a combined approach is recommended.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:16:50