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Titolo:
Influenza B and C virus NEP (NS2) proteins possess nuclear export activities
Autore:
Paragas, J; Talon, J; ONeill, RE; Anderson, DK; Garcia-Sastre, A; Palese, P;
Indirizzi:
NYU, Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA NYU New York NY USA 10029 Sch Med, Dept Microbiol, New York, NY 10029 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 16, volume: 75, anno: 2001,
pagine: 7375 - 7383
SICI:
0022-538X(200108)75:16<7375:IBACVN>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
REV ACTIVATION DOMAIN; MESSENGER-RNA; A VIRUS; VIRAL RIBONUCLEOPROTEINS; HIV REV; EXPRESSION; TRANSPORT; PARTICLES; IDENTIFICATION; HEMAGGLUTININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Palese, P NYU, Mt Sinai Sch Med, Dept Microbiol, 1 Gustave L Levy Pl, New York, NY 10029 USA NYU 1 Gustave L Levy Pl New York NY USA 10029 York, NY 10029 USA
Citazione:
J. Paragas et al., "Influenza B and C virus NEP (NS2) proteins possess nuclear export activities", J VIROLOGY, 75(16), 2001, pp. 7375-7383

Abstract

Nucleocytoplasmic transport of viral ribonucleoproteins (vRNPs) is an essential aspect of the replication cycle for influenza A, B, and C viruses. These viruses replicate and transcribe their genomes in the nuclei of infected cells. During the late stages of infection, vRNPs must be exported from the nucleus to the cytoplasm prior to transport to viral assembly sites on the cellular plasma membrane. Previously, we demonstrated that the influenzaA virus nuclear export protein (NEP, formerly referred to as the NS2 protein) mediates the export of vRNPs. In this report, we suggest that for influenza B and C viruses the nuclear export function is also performed by the orthologous NEP proteins (formerly referred to as the NS2 protein). The influenza virus B and C NEP proteins interact in the yeast two-hybrid assay with a subset of nucleoporins and with the Crm1 nuclear export factor and can functionally replace the effector domain from the human immunodeficiency virus type 1 Rev protein. We established a plasmid transfection system for the generation of virus-like particles (VLPs) in which a functional viral RNA-like chloramphenicol acetyltransferase (CAT) gene is delivered to a new cell. VLPs generated in the absence of the influenza B virus NEP protein wereunable to transfer the viral RNA-like CAT gene to a new cell. From these data, we suggest that the nuclear export of the influenza B and C vRNPs are mediated through interaction between NEP proteins and the cellular nucleocytoplasmic export machinery.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:54:25