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Titolo:
Development of a rapid chromatographic strip test for the pen-side detection of foot-and-mouth disease virus antigen
Autore:
Reid, SM; Ferris, NP; Bruning, A; Hutchings, GH; Kowalska, Z; Akerblom, L;
Indirizzi:
Inst Anim Hlth, Pirbright Lab, Woking GU24 0NF, Surrey, England Inst Anim Hlth Woking Surrey England GU24 0NF g GU24 0NF, Surrey, England SVANOVA Biotech, S-75183 Uppsala, Sweden SVANOVA Biotech Uppsala Sweden S-75183 Biotech, S-75183 Uppsala, Sweden
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 2, volume: 96, anno: 2001,
pagine: 189 - 202
SICI:
0166-0934(200108)96:2<189:DOARCS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; LINKED-IMMUNOSORBENT-ASSAY; CLINICAL-SAMPLES; DIAGNOSIS; DIFFERENTIATION;
Keywords:
rapid diagnosis; foot-and-mouth disease; chromatographic strip test;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Reid, SM Inst Anim Hlth, Pirbright Lab, Ash Rd, Woking GU24 0NF, Surrey, England Inst Anim Hlth Ash Rd Woking Surrey England GU24 0NF rey, England
Citazione:
S.M. Reid et al., "Development of a rapid chromatographic strip test for the pen-side detection of foot-and-mouth disease virus antigen", J VIROL MET, 96(2), 2001, pp. 189-202

Abstract

Foot-and-mouth disease (FMD) is the most contagious animal virus disease of cloven-hoofed livestock and requires reliable and accurate diagnosis for the implementation of measures to control effectively its spread. Routine diagnosis of FMD is carried out at the OIE/FAO World Reference Laboratory for Foot-and-Mouth Disease (WRL for FMD), Pirbright by the combined use of ELISA and virus isolation in cell culture supplemented by reverse transcription polymerase chain reaction (RT-PCR) methods. These techniques require,skilled personnel and dedicated laboratory facilities which are expensive. Thedevelopment of a rapid and simple test for the detection of FMD virus antigen using Clearview (TM) chromatographic strip test technology for field application is described. This device detected FMD viral antigen in nasal swabs, epithelial suspensions and probangs from clinical samples submitted from the field, from animals infected experimentally and in supernatant fluidsresulting from their passage in cell culture. The test system was more sensitive than ELISA for the diagnosis of all seven serotypes of FMD virus in the epithelial suspensions and nasal swabs and had equivalent sensitivity to the ELISA for the detection of contemporary virus strains in cell culturesupernatant fluids. The study demonstrated the potential for this device to confirm a clinical diagnosis at the site of a suspected FMD outbreak, thereby offering the possibility of implementing control procedures more rapidly. Such pen-side diagnosis would have particular benefits in FMD emergencies, relevance to FMD control programmes which operate in endemic regions ofthe world such as South East Asia and for increasing disease awareness in other areas where efforts to control disease may be difficult. In each circumstance the availability of a pen-side device for diagnosis would reduce the necessity for sending routine diagnostic samples to an FMD laboratory and thereby reduce the delay in diagnosis, which can in some areas be considerable. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:31:27