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Titolo:
Functional implications of neurotransmitter expression during axonal regeneration: Serotonin, but not peptides, auto-regulate axon growth of an identified central neuron
Autore:
Koert, CE; Spencer, GE; van Minnen, J; Li, KW; Geraerts, WPM; Syed, NI; Smit, AB; van Kesteren, RE;
Indirizzi:
Vrije Univ Amsterdam, Fac Biol, Dept Mol & Cellular Neurobiol, Neurosci Res Inst, NL-1081 HV Amsterdam, Netherlands Vrije Univ Amsterdam Amsterdam Netherlands NL-1081 HV erdam, Netherlands Univ Calgary, Fac Med, Resp Res Grp, Calgary, AB T2N 4N1, Canada Univ Calgary Calgary AB Canada T2N 4N1 s Grp, Calgary, AB T2N 4N1, Canada Univ Calgary, Fac Med, Neurosci Res Grp, Calgary, AB T2N 4N1, Canada Univ Calgary Calgary AB Canada T2N 4N1 s Grp, Calgary, AB T2N 4N1, Canada
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 15, volume: 21, anno: 2001,
pagine: 5597 - 5606
SICI:
0270-6474(20010801)21:15<5597:FIONED>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL GIANT-CELLS; VASOACTIVE-INTESTINAL-PEPTIDE; CENTRAL PATTERN GENERATOR; CENTRAL-NERVOUS-SYSTEM; LYMNAEA-STAGNALIS; FEEDING SYSTEM; SYMPATHETIC NEURONS; NEURITE OUTGROWTH; MOLLUSK LYMNAEA; MODULATORY ROLE;
Keywords:
neuronal regeneration; neurite outgrowth; synapse formation; behavioral recovery; serotonin; myomodulin; Lymnaea stagnalis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: van Kesteren, RE Vrije Univ Amsterdam, Fac Biol, Dept Mol & Cellular Neurobiol, Neurosci Res Inst, De Boelelaan 1087, NL-1081 HV Amsterdam, Netherlands Vrije Univ Amsterdam De Boelelaan 1087 Amsterdam Netherlands NL-1081 HV
Citazione:
C.E. Koert et al., "Functional implications of neurotransmitter expression during axonal regeneration: Serotonin, but not peptides, auto-regulate axon growth of an identified central neuron", J NEUROSC, 21(15), 2001, pp. 5597-5606

Abstract

We studied the regenerative properties of one of two electrically coupled molluscan neurons, the serotonergic cerebral giant cells (CGCs) of Lymnaea stagnalis, after axotomy. The CGCs play a crucial role in feeding behavior,and when both cells are disconnected from their target neurons, animals nolonger feed. When one CGC was permanently disconnected from its targets and the other was reversibly damaged by a nerve crush, the latter one regenerated over a period of 2 weeks to reform functional synapses with specific target neurons. At the same time, recovery of the feeding behavior was observed. After the crush, neuropeptide gene expression in the CGC was downregulated to similar to 50%. Serotonin synthesis, on the other hand, remained unaffected, suggesting that serotonin might have an active role in regeneration. In primary neuron culture, CGCs failed to extend neurites in the presence of serotonin; in cells that extended neurites in the absence of serotonin, focally applied serotonin, but not neuropeptides, induced growth cone collapse. Using serotonin-sensitive sniffer cells, we show that CGC neurites and growth cones release serotonin in culture. Finally, both the spontaneous and stimulation-induced release of serotonin from CGCs in culture resulted in growth cone collapse responses that could be blocked by the serotonin receptor antagonist methysergide. Our data suggest that auto-released serotonin is inhibitory to CGC neurite outgrowth in vitro. During regeneration in vivo, serotonin release might fine-tune axon guidance and branching by inducing local collapse responses in extending neurites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:31:14