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Titolo:
Single-peak resolution criteria for optimization of mobile phase composition in liquid chromatography
Autore:
Caballero, RD; Lopez-Grio, SJ; Torres-Lapasio, JR; Garcia-Alvarez-Coque, MC;
Indirizzi:
Univ Valencia, Dept Quim Analit, Fac Quim, E-46100 Valencia, Spain Univ Valencia Valencia Spain E-46100 , Fac Quim, E-46100 Valencia, Spain
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 13, volume: 24, anno: 2001,
pagine: 1895 - 1919
SICI:
1082-6076(2001)24:13<1895:SRCFOO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY-ELECTROPHORESIS; SULFONAMIDES; BEHAVIOR; MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Garcia-Alvarez-Coque, MC Univ Valencia, Dept Quim Analit, Fac Quim, E-46100 Valencia, Spain Univ Valencia Valencia Spain E-46100 cia, Spain
Citazione:
R.D. Caballero et al., "Single-peak resolution criteria for optimization of mobile phase composition in liquid chromatography", J LIQ CHR R, 24(13), 2001, pp. 1895-1919

Abstract

Three criteria that evaluate the single peak resolution (peak purity) in chromatography, the free height fraction, free area fraction, and valley ratio, are examined. The main advantages of these criteria against other criteria based on peak pair separation, are that the measurements are not affected by the identity of neighboring peaks and are normalized, which make themvery intuitive. The methodology is illustrated through the isocratic separation of mixtures of several sulphonamides (sulphacetamide, sulphadiazine, sulphadimethoxine, sulphaguanidine, sulphamerazine, sulphamethazine, sulphamethoxazole, sulphamethizole, sulphamonomethoxine, sulphanilamide, sulphapyridine, sulphaquinoxaline, and sulphathiazole). Among the three proposed criteria, the measurement of free area fractions gave the best description of the resolution, independent of the overlappingdegree of the chromatographic peaks. The usefulness of this criterion for the resolution of individual peaks in a complex mixture is shown. The best separation conditions are reached easily for the individual compounds, and take into account peak shape and size.

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Documento generato il 29/03/20 alle ore 17:37:01