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Titolo:
Identification of rab7 as a melanosome-associated protein involved in the intracellular transport of tyrosinase-related protein 1
Autore:
Gomez, PF; Luo, D; Hirosaki, K; Shinoda, K; Yamashita, T; Suzuki, J; Otsu, K; Ishikawa, K; Jimbow, K;
Indirizzi:
Sapporo Med Univ, Sch Med, Dept Dermatol, Chuo Univ, Sapporo, Hokkaido 0608543, Japan Sapporo Med Univ Sapporo Hokkaido Japan 0608543 , Hokkaido 0608543, Japan Univ Alberta, Div Dermatol & Cutaneous Sci, Edmonton, AB T6G 2E1, Canada Univ Alberta Edmonton AB Canada T6G 2E1 Sci, Edmonton, AB T6G 2E1, Canada Yamagata Univ, Sch Med, Dept Biochem, Yamagata 99023, Japan Yamagata UnivYamagata Japan 99023 , Dept Biochem, Yamagata 99023, Japan
Titolo Testata:
JOURNAL OF INVESTIGATIVE DERMATOLOGY
fascicolo: 1, volume: 117, anno: 2001,
pagine: 81 - 90
SICI:
0022-202X(200107)117:1<81:IORAAM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING PROTEIN; HERMANSKY-PUDLAK-SYNDROME; CHEDIAK-HIGASHI-SYNDROME; STORAGE POOL DEFICIENCY; DOPACHROME TAUTOMERASE; ENDOCYTIC COMPARTMENTS; NEOPLASTIC MELANOCYTES; OXIDASE ACTIVITY; GENE-EXPRESSION; PLASMA-MEMBRANE;
Keywords:
intracellular transport; melanogenesis; melanosomes; rab7; small GTP-binding proteins; tyrosinase-related protein 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
85
Recensione:
Indirizzi per estratti:
Indirizzo: Jimbow, K Sapporo Med Univ, Sch Med, Dept Dermatol, Chuo Univ, S-1,W-16, Sapporo, Hokkaido 0608543, Japan Sapporo Med Univ S-1,W-16 Sapporo Hokkaido Japan 0608543 , Japan
Citazione:
P.F. Gomez et al., "Identification of rab7 as a melanosome-associated protein involved in the intracellular transport of tyrosinase-related protein 1", J INVES DER, 117(1), 2001, pp. 81-90

Abstract

The melanosome is a unique secretary granule of the melanocyte in which melanin pigments are synthesized by tyrosinase gene family glycoproteins, Melanogenesis is a highly regulated process because of its inherent toxicity. An understanding of the various regulatory mechanisms is important in delineating the pathophysiology involved in pigmentary disorders and melanoma, We have purified and analyzed the total melanosomal proteins from B16 mouse melanoma tumors in order to identify new proteins that may be involved in the control of the melanogenesis process, Melanosomal proteins were resolvedby two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, a predominant spot (27 kDa with isoelectric point 5.8-6.4) was excised and digested with cyanogen bromide, and the fragments were sequenced. Synthetic oligonucleotide primers were synthesized corresponding to the peptide sequences, and reverse transcriptase polymerase chain reaction amplification of total RNA from B16 cells was carried out, Sequencing of one of the polymerase-chain-reaction-mediated clones demonstrated 80%-97% sequence homology of 200 bp nucleotide with GTP-binding proteins at the 3'-untranslated region. GTP-binding assay on two-dimensional gels of melanosomal proteins showed the presence of several (five to six) small GTP-binding proteins, suggesting that small GTP-binding proteins are associated with the melanosome, Among the known GTP-binding proteins with similar molecular weight and isoelectric point ranges, rab3, rab7, and rab8 were found to be present in the melanosomal fraction by immunoblotting. Confocal immunofluorescence microscopy showed that rab7 is colocalized with the tyrosinase-related protein 1 around the perinuclear area as well as, in part, in the perikaryon, thereby suggesting that rab7 might be involved in the intracellular transport of tyrosinase-related protein 1. Tyrosinase-related protein 1 transport was blocked by the treatment of B16 cells with antisense oligonucleotide to rab7, Wesuggest (i) that rab7 is a melanosome-associated molecule, (ii) that tyrosinase-related protein 1 is present in late-endosome delineated granules, and (iii) that rab7 is involved in the transport of tyrosinase-related protein 1 from the late-endosome delineated granule to the melanosome.

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Documento generato il 01/10/20 alle ore 07:37:36