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Titolo:
Preparation and optimization of bienzyme multilayer films using lectin andglyco-enzymes for biosensor applications
Autore:
Kobayashi, Y; Anzai, J;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Ku, Sendai, Miyagi 9808578, Japan
Titolo Testata:
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
fascicolo: 1-2, volume: 507, anno: 2001,
pagine: 250 - 255
Fonte:
ISI
Lingua:
ENG
Soggetto:
AVIDIN-BIOTIN SYSTEM; MODIFIED ELECTRODES; HYDROGEN-PEROXIDE; GLUCOSE-OXIDASE; THIN-FILMS; SENSORS; CONSTRUCTION; DEPOSITION; LACTATE; ACID;
Keywords:
enzyme sensor; multilayer film; lectin; concanavalin A; glyco-enzyme; bienzyme sensor; hydrogen peroxide sensors; glucose sensors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Anzai, J Tohoku Univ, Grad Sch Pharmaceut Sci, Aoba Ku, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 i, Miyagi 9808578, Japan
Citazione:
Y. Kobayashi e J. Anzai, "Preparation and optimization of bienzyme multilayer films using lectin andglyco-enzymes for biosensor applications", J ELEC CHEM, 507(1-2), 2001, pp. 250-255

Abstract

Enzyme multilayer films were prepared on the surface of a glassy carbon electrode by a layer-by-layer deposition of concanavalin A (Con A) and horseradish peroxidase (HRP) or glucose oxidase (GOx) alternately. The enzyme thin films were formed through biological affinity between Con A and sugar chains intrinsically located on the surface of the enzymes. Both HRP and GOx retained their catalytic activity in the multilayer film and the enzyme-modified electrodes served as enzyme biosensors. For the glucose sensors prepared using HRP-GOx bienzyme multilayer films, the loading and geometry of theenzymes in the multilayer film were optimized by varying the number of enzyme layers and the sequence of immobilization in the multilayer film. The magnitude of response of the glucose sensors increased with the increasing number of Cox layers, while the bilayers of HRP sufficed for attaining maximum response. It was found that the electrode surface should be first coatedwith HRP layers and then the GOx layer should be immobilized as an outer layer to obtain higher performance sensors. The results were explained reasonably based on the relative activity of the enzymes and the reaction scheme. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:52:50