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Titolo:
Rab7 regulates phagosome maturation in Dictyostelium
Autore:
Rupper, A; Grove, B; Cardelli, J;
Indirizzi:
LSUHSC, Dept Microbiol & Immunol, Shreveport, LA 71130 USA LSUHSC Shreveport LA USA 71130 robiol & Immunol, Shreveport, LA 71130 USA LSUHSC, Feist Weiller Canc Ctr, Shreveport, LA 71130 USA LSUHSC Shreveport LA USA 71130 Weiller Canc Ctr, Shreveport, LA 71130 USA Univ N Dakota, Dept Anat & Cell Biol, Grand Forks, ND 58202 USA Univ N Dakota Grand Forks ND USA 58202 ll Biol, Grand Forks, ND 58202 USA
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 13, volume: 114, anno: 2001,
pagine: 2449 - 2460
SICI:
0021-9533(200107)114:13<2449:RRPMID>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN RAB4; ENDOCYTIC PATHWAY; PROCATHEPSIN-B; LATE ENDOSOMES; SMALL GTPASE; SUGAR MODIFICATIONS; J774 MACROPHAGES; DISCOIDEUM; FUSION; LYSOSOMES;
Keywords:
Rab7; phagocytosis; phagosome maturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Cardelli, J LSUHSC, Dept Microbiol & Immunol, Shreveport, LA 71130 USA LSUHSC Shreveport LA USA 71130 unol, Shreveport, LA 71130 USA
Citazione:
A. Rupper et al., "Rab7 regulates phagosome maturation in Dictyostelium", J CELL SCI, 114(13), 2001, pp. 2449-2460

Abstract

A Dictyostelium Rab7 homolog has been demonstrated to regulate fluid-phaseinflux, efflux, retention of lysosomal hydrolases and phagocytosis. Since Rab7 function appeared to be required for efficient phagocytosis, we soughtto further characterize the role of Rab7 in phagosomal maturation. Expression of GFP-Rab7 resulted in labeling of both early and late phagosomes containing yeast, but not forming phagocytic cups. In order to determine if Rab7 played a role in regulating membrane traffic between the endo/lysosomal system and maturing phagosomes, latex bead containing (LBC) phagosomes were purified from wild-type cells at various times after internalization. Glycosidases, cysteine proteinases, Rab7 and lysosomally associated membrane proteins were delivered rapidly to nascent phagosomes in control cells. LBC phagosomes isolated from cells overexpressing dominant negative (DN) Rab7 contained very low levels of LmpA (lysosomal integral membrane protein) and alpha -mannosidase was not detectable. Interestingly, cysteine proteinases were delivered to phagosomes as apparent proforms in cells overexpressing DN Rab7. Despite these defects, phagosomes in cells overexpressing DN Rab7 matured to form multi-particle spacious phagosomes, except that these phagosomes remained significantly more acidic than control phagosomes. These results suggested that Rab7 regulates both an early and late steps of phagosomal maturation, similar to its role in the endo/lysosomal system.

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Documento generato il 01/10/20 alle ore 06:01:15