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Titolo:
Action of an HMG CoA reductase inhibitor, lovastatin, on apoptosis of untransformed and ts-SV40 transformed human smooth muscle cells derived from saphenous vein
Autore:
Unlu, S; Clunn, G; Schachter, M; Demoliou-Mason, C; Hughes, AD;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, St Marys Hosp, London W2 1NY, England Univ London Imperial Coll Sci Technol & Med London England W2 1NY ngland
Titolo Testata:
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
fascicolo: 2, volume: 38, anno: 2001,
pagine: 161 - 173
SICI:
0160-2446(200108)38:2<161:AOAHCR>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
AVERAGE CHOLESTEROL LEVELS; MEVALONATE PATHWAY; IN-VITRO; CORONARY EVENTS; FLOW-CYTOMETRY; CDC42 GTPASES; PROLIFERATION; RHO; ATHEROSCLEROSIS; P21(WAF1/CIP1);
Keywords:
HMG CoA reductase; lovastatin; apoptosis; proliferation; vascular smooth muscle; SV40;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Hughes, AD Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, St Marys Hosp, QEQM Wing,S Wharf Rd, London W2 1NY, England Univ London Imperial Coll Sci Technol & Med QEQM Wing,S Wharf Rd London England W2 1NY
Citazione:
S. Unlu et al., "Action of an HMG CoA reductase inhibitor, lovastatin, on apoptosis of untransformed and ts-SV40 transformed human smooth muscle cells derived from saphenous vein", J CARDIO PH, 38(2), 2001, pp. 161-173

Abstract

The effect of lovastatin, an inhibitor of 3-hydroxymethyl-3-glutaryl coenzyme A (HMG CoA) reductase, was examined on human vascular smooth muscle cells (HVSMC). Untransformed HVSMC were obtained from saphenous vein and in addition an SV-40 transformed immortalized cell line (HVTs-SM1) derived from saphenous vein smooth muscle was also used. HVTs-SM1 cell proliferation andDNA synthesis were measured, and cell cycle analysis was performed by flowcytometry. Apoptosis in both cell types was assessed by a combination of flow cytometry, terminal deoxynucleotidyl transferase (TUNEL) reagent-based immunocytochemistry, DAPI staining, and DNA agarose gel electrophoresis. Lovastatin had no effect on apoptosis of HVSMC over 96 h in serum-free conditions or after stimulation with platelet-derived growth factor (PDGF-BB), although PDGF-BB increased apoptosis in HVSMC, and this was prevented by lovastatin. In HVTs-SM1 cells lovastatin inhibited cell proliferation and DNA synthesis and induced apoptosis in a time- and concentration-dependent manner. The effects of lovastatin on cell proliferation, DNA synthesis, and apoptosis were prevented by coincubation with mevalonate and geranylgeranyl pyrophosphate, but not by farnesyl pyrophosphate. Lovastatin does not induce apoptosis in saphenous vein HVSMC in culture and inhibits PDGF-BB-induced DNA synthesis and apoptosis. In contrast, in SV40 transformed immortalized HVTs-SM1 cells, lovastatin induces apoptosis and inhibits cell proliferation and DNA synthesis. The pro-apoptotic effects of lovastatin in SV40 transformed HVTs-SM1 cells may be related to the enhanced rate of proliferation or deregulation of the cell cycle in this cell line.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 01:33:50