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Titolo:
The motility of demembranated human spermatozoa is inhibited by free calcium ion activities of 500 nmol/L or more
Autore:
Williams, KM; Ford, WCL;
Indirizzi:
Univ Bristol, St Michaels Hosp, Div Obstet & Gynaecol, Bristol BS2 8EG, Avon, England Univ Bristol Bristol Avon England BS2 8EG Bristol BS2 8EG, Avon, England
Titolo Testata:
INTERNATIONAL JOURNAL OF ANDROLOGY
fascicolo: 4, volume: 24, anno: 2001,
pagine: 216 - 224
SICI:
0105-6263(200108)24:4<216:TMODHS>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOGEN-SYNTHASE KINASE-3; HUMAN SPERM MOTILITY; SEA-URCHIN SPERM; INTRACELLULAR CALCIUM; HAMSTER SPERM; CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE; PROTEIN-PHOSPHORYLATION; BOVINE SPERMATOZOA; REGULATORY SUBUNIT; EPIDIDYMAL SPERM;
Keywords:
calcium; cryopreservation; demembranation; human spermatozoa; motility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Ford, WCL Univ Bristol, St Michaels Hosp, Div Obstet & Gynaecol, SouthwellSt, Bristol BS2 8EG, Avon, England Univ Bristol Southwell St Bristol Avon England BS2 8EG , England
Citazione:
K.M. Williams e W.C.L. Ford, "The motility of demembranated human spermatozoa is inhibited by free calcium ion activities of 500 nmol/L or more", INT J ANDR, 24(4), 2001, pp. 216-224

Abstract

A number of studies have demonstrated that high calcium ion activities inhibit sperm motility, but little is known about the effect of different calcium activities close to the physiological range. Therefore, we investigatedwhether raising calcium activities within the submicromolar range would inhibit the motility of demembranated human spermatozoa. Spermatozoa were demembranated with Triton X-100 and motility was measured objectively by computer assisted semen analysis. Motility, reactivated by 1 mol adenosine 5'-triphosphate (A TP)/L, was short lived, with maximum activity only sustained for about 1 min. Reactivated motility was not affected by 50 mu mol cAMP/L. The amplitude of lateral head displacement was significantly greater at room temperature than at 37 degreesC, but there were no significant differences between the percentage of sperm motile or their velocity at the two temperatures. The calcium buffer 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) at 1 mmol/L was included in the demembranation-reactivation medium, and free calcium ion activities were calibrated using the fluorescent calcium probe Fura-2. Calcium ion activities of greater than or equal to 500 nmol/L significantly inhibited the percentage of demembranated-reactivated spermatozoa that were motile, and the velocity and lateral head displacement of these cells. The range of intracellular calcium activities inspermatozoa from 24 cryopreserved ejaculates was 110-534 nmol/L; roughly twice the value in fresh spermatozoa. Therefore, calcium ion activities in the range observed in cryopreserved spermatozoa can inhibit the activity of demembranated human spermatozoa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:27:57