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Titolo:
Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion
Autore:
Lourbakos, A; Potempa, J; Travis, J; DAndrea, MR; Andrade-Gordon, P; Santulli, R; Mackie, EJ; Pike, RN;
Indirizzi:
Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 Biol, Clayton, Vic 3800, Australia Univ Melbourne, Sch Vet Sci, Parkville, Vic 3010, Australia Univ Melbourne Parkville Vic Australia 3010 arkville, Vic 3010, Australia Jagiellonian Univ, Dept Microbiol & Immunol, Krakow, Poland Jagiellonian Univ Krakow Poland ept Microbiol & Immunol, Krakow, Poland Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA Univ Georgia Athens GA USA 30602 Biochem & Mol Biol, Athens, GA 30602 USA RW Johnson Pharmaceut Res Inst, Spring House, PA 19477 USA RW Johnson Pharmaceut Res Inst Spring House PA USA 19477 se, PA 19477 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 8, volume: 69, anno: 2001,
pagine: 5121 - 5130
SICI:
0019-9567(200108)69:8<5121:APFPGA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
DESTRUCTIVE PERIODONTAL-DISEASE; HUMAN ENDOTHELIAL-CELLS; THROMBIN RECEPTOR; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; BACTEROIDES-GINGIVALIS; PROINFLAMMATORY CYTOKINES; REFRACTORY PERIODONTITIS; CYSTEINE PROTEINASES; MOLECULAR-CLONING; CREVICULAR FLUID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Pike, RN Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia Monash Univ Clayton Vic Australia 3800 yton, Vic 3800, Australia
Citazione:
A. Lourbakos et al., "Arginine-specific protease from Porphyromonas gingivalis activates protease-activated receptors on human oral epithelial cells and induces interleukin-6 secretion", INFEC IMMUN, 69(8), 2001, pp. 5121-5130

Abstract

Periodontitis is a chronic inflammatory disease affecting oral tissues. Oral epithelial cells represent the primary barrier against bacteria causing the disease. We examined the responses of such cells to an arginine-specific cysteine proteinase (RgpB) produced by a causative agent of periodontal disease, Porphyromonas gingivalis. This protease caused an intracellular calcium transient in an oral epithelial cell line (KB), which was dependent onits enzymatic activity. Since protease-activated receptors (PARs) might mediate such signaling, reverse transcription-PCR was used to characterize the range of these receptors expressed in the KB cells. The cells were found to express PAR-1, PAR-2, and PAR-3, but not PAR-4. In immunohistochemical studies, human gingival epithelial cells were found to express PAR-1, PAR-2,and PAR-3 on their surface, but not PAR-4, indicating that the cell line was an effective model for the in vivo situation. PAR-1 and PAR-2 expressionwas confirmed in intracellular calcium mobilization assays by treatment ofthe cells with the relevant receptor agonist peptides. Desensitization experiments strongly indicated that signaling of the effects of RgpB was occurring through PAR-1 and PAR-2. Studies with cells individually transfected with each of these two receptors confirmed that they were both activated by RgpB. Finally, it was shown that, in the oral epithelial cell line, PAR activation by the bacterial protease-stimulated secretion of interleukin-6. This induction of a powerful proinflammatory cytokine suggests a mechanism whereby cysteine proteases from P. gingivalis might mediate inflammatory events associated with periodontal disease on first contact with a primary barrier of cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 01:19:11