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Titolo:
Stress-induced ClpP serine protease of Listeria monocytogenes is essentialfor induction of listeriolysin O-dependent protective immunity
Autore:
Gaillot, O; Bregenholt, S; Jaubert, F; Di Santo, JP; Berche, P;
Indirizzi:
CHU Necker Enfants Malad, INSERM, U411, F-75730 Paris, France CHU Necker Enfants Malad Paris France F-75730 411, F-75730 Paris, France CHU Necker Enfants Malad, INSERM, U429, F-75730 Paris, France CHU Necker Enfants Malad Paris France F-75730 429, F-75730 Paris, France CHU Necker Enfants Malad, Serv Anat Pathol, F-75730 Paris, France CHU Necker Enfants Malad Paris France F-75730 hol, F-75730 Paris, France Inst Pasteur, Unite Cytokines & Dev Lymphoide, Paris, France Inst PasteurParis France nite Cytokines & Dev Lymphoide, Paris, France
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 8, volume: 69, anno: 2001,
pagine: 4938 - 4943
SICI:
0019-9567(200108)69:8<4938:SCSPOL>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-MEDIATED IMMUNITY; CLASS-I MOLECULES; T-CELLS; INTRACELLULAR-LOCALIZATION; ANTILISTERIAL IMMUNITY; INFECTED-CELLS; VIRULENCE; HEMOLYSIN; GROWTH; MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Berche, P Fac Med Necker Enfants Malad, INSERM, U411, 156 Rue de Vaugirard, F-75730 Paris 15, France Fac Med Necker Enfants Malad 156 Rue de Vaugirard Paris France 15
Citazione:
O. Gaillot et al., "Stress-induced ClpP serine protease of Listeria monocytogenes is essentialfor induction of listeriolysin O-dependent protective immunity", INFEC IMMUN, 69(8), 2001, pp. 4938-4943

Abstract

The stress-induced protease ClpP is required for virulence of the facultative intracellular pathogen Listeria monocytogenes. We previously found thatin the absence of ClpP, the virulence of this pathogen was strongly reduced, mainly due to the decreased production of functional listeriolysin O (LLO), a major immunodominant virulence factor promoting intracellular growth. In this work, a clpP deletion mutant of L. monocytogenes was used to studythe generation of anti-Listeria protective immunity. We found that ClpP isrequired for the intracellular growth of L. monocytogenes in resident macrophages in vivo. Mice infected with doses as high as 10(6) clpP mutant bacteria were not protected against a lethal challenge of wild-type bacteria and did not develop any detectable LLO-specific cytolytic T cells or antibodies, suggesting that the amount of LLO produced in infected mice under theseconditions was too low to induce a specific immune response. However, in contrast to the results obtained with a mutant with a disrupted hly gene, this lack of protection was overcome by inoculation of very high infecting doses of clpP mutant bacteria (5 x 10(8)), thus producing sufficient amounts of LLO to stimulate anti-Listeria immunity. The role of ClpP was confirmed by showing that anti-Listeria immunity was restored in mice infected with aclpP-complemented mutant. These results indicate that the stress-induced serine protease ClpP is a potential target for modulating the presentation of protective antigens such as LLO and thereby the immune response against L. monocytogenes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 16:05:51