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Titolo:
IN-VITRO AND IN-VIVO SPECTROFLUOROMETRY OF A WATER-SOLUBLE META-(TETRAHYDROXYPHENYL)CHLORIN (M-THPC) DERIVATIVE
Autore:
MORLET L; VONARX V; FOULTIER MT; GOUYETTE A; STEWART C; LENZ P; PATRICE T;
Indirizzi:
FAC PHARM,6EME ETAGE F-44035 NANTES FRANCE FAC PHARM F-44035 NANTES FRANCE SCOTIA DRUG DISCOVERY GUILDFORD GU1 1BA SURREY ENGLAND INSERM U281 F-69424 LYON FRANCE
Titolo Testata:
Journal of photochemistry and photobiology.B, Biology
fascicolo: 3, volume: 39, anno: 1997,
pagine: 249 - 257
SICI:
1011-1344(1997)39:3<249:IAISOA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOTODYNAMIC THERAPY; MALIGNANT MESOTHELIOMA; PHOTOSENSITIZERS; BIODISTRIBUTION; FLUORESCENCE; TUMORS; MICE;
Keywords:
CHLORIN; MICROSPECTROFLUOROMETRY; OPTICAL FIBER SPECTROFLUOROMETER; PHOTODYNAMIC THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
L. Morlet et al., "IN-VITRO AND IN-VIVO SPECTROFLUOROMETRY OF A WATER-SOLUBLE META-(TETRAHYDROXYPHENYL)CHLORIN (M-THPC) DERIVATIVE", Journal of photochemistry and photobiology.B, Biology, 39(3), 1997, pp. 249-257

Abstract

The pharmacokinetics of a water-soluble derivative obtained from meta-(tetrahydroxyphenyl) chlorin (m-THPC) was evaluated in in vitro and in vivo studies, Cytoplasm fluorescence was measured in two cell models(L1210 and HT29) using a flow cytometer and a confocal microspectrofluorometer. Cells were incubated with the compound at several doses (0-150 mu g ml(-1) for flow cytometry) and for several time periods (0-6 h for microspectrofluorometry). For in vivo studies, nude mice were grafted with human adenocarcinoma 15 days before intraperitoneal injection of polyethylene glycol-m-THPC (PEG-m-THPC). Fluorescence was recorded through an optical fibre spectrofluorometer using the 660 nm peak for detection. In in vitro studies, the fluorescence was found to be proportional to the dose. Maximum fluorescence was recorded in L1210 cells earlier and more intensely than in HT29 cells (3 h at 202 +/- 14 counts s(-1) and 5 h at 43 +/- 2.15 counts s(-1) respectively). Concerning in vivo studies, maximum tumour fluorescence was observed 24 h after injection (3568 +/- 178 counts s(-1)). Selectivity was expressed by the calculated tumour-to-skin and tumour-to-muscle ratios. The time taken to observe the maximum ratios (2.95 +/- 0.16 for tumour-to-skin and 6.61 +/- 0.3 for tumour-to-muscle) was almost the same as the time taken to observe the maximum fluorescence in the tumour. Studies are inprogress to correlate these results with photodynamic effects. (C) 1997 Elsevier Science S.A.

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Documento generato il 25/11/20 alle ore 04:20:03