Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Hedgehog signalling is required for maintenance of myf5 and myoD expression and timely terminal differentiation in zebrafish adaxial myogenesis
Autore:
Coutelle, O; Blagden, CS; Hampson, R; Halai, C; Rigby, PWJ; Hughes, SM;
Indirizzi:
Univ London Kings Coll, MRC, Muscle & Cell Motil Unit, London SE1 1UL, England Univ London Kings Coll London England SE1 1UL t, London SE1 1UL, England Natl Inst Med Res, MRC, Div Eukaryot Mol Genet, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA Genet, London NW7 1AA, England
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 236, anno: 2001,
pagine: 136 - 150
SICI:
0012-1606(20010801)236:1<136:HSIRFM>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUSCLE-CELL-TYPE; SONIC-HEDGEHOG; FLOOR PLATE; SOMITE FORMATION; SKELETAL-MUSCLE; DANIO-RERIO; NO TAIL; ECTOPIC EXPRESSION; PRECURSOR CELLS; MOUSE EMBRYO;
Keywords:
skeletal muscle; zebrafish; myf5; myoD; hedgehog; differentiation; somite; myoblast; myogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Hughes, SM Univ London Kings Coll, MRC, Muscle & Cell Motil Unit, 4th Floor S,New Hunts House,Guys Campus, London SE1 1UL, England Univ London Kings Coll 4th Floor S,New Hunts House,Guys Campus London England SE1 1UL
Citazione:
O. Coutelle et al., "Hedgehog signalling is required for maintenance of myf5 and myoD expression and timely terminal differentiation in zebrafish adaxial myogenesis", DEVELOP BIO, 236(1), 2001, pp. 136-150

Abstract

Hedgehog proteins have been implicated in the control of myogenesis in themedial vertebrate somite. In the mouse, normal epaxial expression of the myogenic transcription factor gene myf5 is dependent on Sonic hedgehog. Herewe examine in zebrafish the interaction between Hedgehog signals, the expression of myoD family genes, including the newly cloned zebrafish myf5, andslow myogenesis. We show that Sonic hedgehog is necessary for normal expression of both myf5 and myoD in adaxial slow muscle precursors, but not in lateral paraxial mesoderm. Expression of both genes is initiated normally inrostral presomitic mesoderm in sonic you mutants, which lack all Sonic hedgehog. Similar initiation continues during tailbud outgrowth when the cellsforming caudal somites are generated. However, adaxial cells in sonic you embryos are delayed in terminal differentiation and caudal adaxial cells fail to maintain myogenic regulatory factor expression. Despite these defects, other signals are able to maintain, or reinitiate, some slow muscle development in sonic you mutants. In the cyclops mutant, the absence of floorplate-derived Tiggywinkle hedgehog and Sonic hedgehog has no discernible effect on slow adaxial myogenesis. Similarly, the absence of notochord-derived Sonic hedgehog and Echidna hedgehog in mutants lacking notochord delays, butdoes not prevent, adaxial slow muscle development. In contrast, removal ofboth Sonic hedgehog and a floorplate signal, probably Tiggywinkle hedgehog, from the embryonic midline in cyclops;sonic you double mutants essentially abolishes slow myogenesis. We conclude that several midline signals, likely to be various Hedgehogs, collaborate to maintain adaxial slow myogenesisin the zebrafish embryo. Moreover, the data demonstrate that, in the absence of this required Hedgehog signalling, expression of myf5 and myoD is insufficient to commit cells to adaxial myogenesis. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 21:00:32