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Titolo:
Prospective clinical study comparing DNA flow cytometry and HPV typing as predictive tests for persistence and progression of CINI/II
Autore:
Melsheimer, P; Klaes, R; Doeberitz, MV; Bastert, G;
Indirizzi:
Univ Heidelberg, Dept Obstet Gynecol & Gynecol Oncol, Heidelberg, Germany Univ Heidelberg Heidelberg Germany & Gynecol Oncol, Heidelberg, Germany Univ Heidelberg, Div Mol Diagnost & Therapy, Heidelberg, Germany Univ Heidelberg Heidelberg Germany gnost & Therapy, Heidelberg, Germany
Titolo Testata:
CYTOMETRY
fascicolo: 3, volume: 46, anno: 2001,
pagine: 166 - 171
SICI:
0196-4763(20010615)46:3<166:PCSCDF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CERVICAL INTRAEPITHELIAL NEOPLASIA; HUMAN PAPILLOMAVIRUS INFECTION; CANCER; HYBRIDIZATION; MALIGNANCY; DIAGNOSIS; GRADE;
Keywords:
cervical intrepithelial neoplasia; DNA ploidy; human papillomavirus; flow cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Melsheimer, P Univ Heidelberg, Dept Obstet & Gynecol, Frauenklin, Vobstr 9, D-69115 Heidelberg, Germany Univ Heidelberg Vobstr 9 Heidelberg Germany D-69115 Germany
Citazione:
P. Melsheimer et al., "Prospective clinical study comparing DNA flow cytometry and HPV typing as predictive tests for persistence and progression of CINI/II", CYTOMETRY, 46(3), 2001, pp. 166-171

Abstract

A cohort of 70 consecutive women at a university hospital colposcopy clinic with untreated CIN I and CIN II (CIN VII) confirmed by cytology and histology was followed for 1 year in the setting of a prospective trial. In the lesions, the presence of DNA from HPV types was examined by restriction fragment length polymorphism (RFLP) analysis. Aneuploid cell lines were demonstrated by aneuploid histograms generated by high-resolution DNA flow cytometry. HPV type 16 infection and the existence of aneuploid cell lines provedto be significant risk factors for CIN VII lesions to persist or progress to CIN III in the 1-year follow-up period in the same cohort of patients. The relative risks and 95% confidence intervals (CI) were 1,81 (1.44-2.76) for aneuploid cell lines and 1,74 (1.10-2.76) for HPV type 16 infection in CIN VII lesions. As a predictive diagnostic test for CIN I/II lesions to persist or progress, the specificity and positive predictive value (PPV) for aneuploid histograms were 100% (CI, 73.5-100%) and 100% (CI, 86,8-100%), respectively. The low sensitivity of 27.3% (CI, 14.9-42.8%) restricted the clinical application of the test, leaving 32 of 44 women with persisting or progressing CINI/II with diploid histograms. HPV type 16 positivity by FRLP had a PPV of 68.4% (CI, 43.5-87.4%) as a prognostic test. Six of 19 HPV 16 infected women showed complete remission of their CIN lesion. A combination of the two tests did not provide any additional information. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:30:27